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Updated: Sep 11, 2025

Molecular Analysis of Endothelial-mesenchymal Transition Induced by Transforming Growth Factor-β Signaling
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Silencing MMP2-AS1 inhibits the proliferation and migration of endothelial cells via YB-1

Zhetao Li1, Kuo Wang2, Ziliang Li3

  • 1Heart Center, Qingdao Hiser Hospital Affiliated of Qingdao University (Qingdao Traditional Chinese Medicine Hospital), Qingdao, China.

Science Progress
|August 12, 2025
PubMed

Insights

Long noncoding RNA MMP2-AS1 promotes atherosclerosis by enhancing endothelial cell proliferation and migration via the YB-1 pathway. Targeting this MMP2-AS1/YB-1 axis may offer new atherosclerosis treatments.

Area of Science:

  • Cardiovascular Biology
  • Molecular Biology
  • RNA Biology

Background:

  • Endothelial cell proliferation and migration are crucial in atherosclerosis development.
  • Long noncoding RNAs (lncRNAs) are implicated in atherosclerosis, affecting endothelial cell functions.
  • The specific role of lncRNA matrix metalloproteinase 2 antisense RNA 1 (MMP2-AS1) in atherosclerosis is not fully understood.

Purpose of the Study:

  • To investigate the molecular mechanism of lncRNA MMP2-AS1 in endothelial cells.
  • To explore the role of the MMP2-AS1/YB-1 signaling pathway in atherosclerosis.
  • To determine if MMP2-AS1 regulates human umbilical vein endothelial cell (HUVEC) proliferation and migration.

Main Methods:

  • Established a HUVEC model using transforming growth factor-β1, oxidized low-density lipoprotein, and hypoxia.
  • Measured the expression of lncRNA MMP2-AS1 under these conditions.
  • Investigated the effect of MMP2-AS1 knockdown on HUVEC proliferation and migration, focusing on YB-1 interaction.

Main Results:

  • Conditions mimicking atherosclerosis induced upregulation of lncRNA MMP2-AS1 expression in HUVECs.
  • Knockdown of MMP2-AS1 significantly reduced HUVEC proliferation and migration.
  • MMP2-AS1 was found to regulate HUVEC proliferation and migration by targeting YB-1.

Conclusions:

  • The MMP2-AS1/YB-1 signaling pathway plays a significant role in regulating endothelial cell behavior in atherosclerosis.
  • MMP2-AS1 promotes HUVEC proliferation and migration through YB-1 targeting.
  • The MMP2-AS1/YB-1 axis represents a potential therapeutic target for treating atherosclerosis.
Keywords:
HUVECsMMP2-AS1YB-1atherosclerosiscell proliferation and migration

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