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Updated: Sep 11, 2025

Isolation of Labile Multi-protein Complexes by in vivo Controlled Cellular Cross-Linking and Immuno-magnetic Affinity Chromatography
Published on: March 9, 2010
Preparation, characterization, and application of camellianin A/soy protein isolate covalent complexes
Peng Gao1, Yuanyang Nie2, Yingxuan Zhou2
1College of Food Science and Engineering, Northwest A&F University, Yangling, China.
Abstract:
Alkaline-assisted processing facilitated the development of camellianin A (CA)-soy protein isolate (SPI) conjugates, with systematic characterization of polyphenol incorporation effects on macromolecular architecture and emulsion stabilization capacity. Multi-spectroscopic profiling confirmed CA-induced structural reorganization of SPI matrices, verifying conjugate formation. Dose-dependent enhancement patterns were observed, where increased CA loading positively correlated with total phenolic content, surface hydrophilicity, ABTS radical scavenging activity and reducing power. The derived nanoemulsions demonstrated superior oxidative stabilization capacity and storage stability, outperforming SPI-only stabilized systems. Our results can advance the utilization of CA in functional foods, providing practical guidance for developing novel food-grade nanoemulsions with enhanced delivery efficacy.
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