Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Characterization of Electrophysiological and Transcriptomic Alterations in Patient-Derived Neurons from CHAMP1 Syndrome.

bioRxiv : the preprint server for biology·2026
Same author

Corrigendum to "Primary and Immortalized Microglia Exhibit Divergent Responses to Hemoglobin" [Experimental Neurology 403 (2026) 115838].

Experimental neurology·2026
Same author

Region-specific cortico-striatal transcriptomic remodeling following early postnatal dopaminergic disturbance.

bioRxiv : the preprint server for biology·2026
Same author

Primary and immortalized microglia exhibit divergent responses to hemoglobin.

Experimental neurology·2026
Same author

Spatial, temporal and sex specific mitochondrial dynamic changes in severe controlled cortical impact mouse model of traumatic brain injury.

bioRxiv : the preprint server for biology·2026
Same author

Copper deficiency impairs oligodendrocyte maturation and social behavior via mitophagy and mTOR suppression in ASD.

Science advances·2026

Related Experiment Video

Updated: Jul 20, 2026

Modeling Encephalopathy of Prematurity Using Prenatal Hypoxia-ischemia with Intra-amniotic Lipopolysaccharide in Rats
07:36

Modeling Encephalopathy of Prematurity Using Prenatal Hypoxia-ischemia with Intra-amniotic Lipopolysaccharide in Rats

Published on: November 20, 2015

11.5K

Developmental Stage-Dependent Transcriptomic Responses to Neonatal Intraventricular Hemorrhage.

Elizabeth Wallace-Anthony1,2, Miriam Zamorano3,4, Hemendra J Vekaria3

  • 1Bioinformatics Core, Department of Neuroscience, Medical University of South Carolina, Charleston, 29425, SC, USA.

Biorxiv : the Preprint Server for Biology
|August 12, 2025
PubMed
Summary

Neonatal intraventricular hemorrhage (IVH) impacts preterm infants. This study reveals that the brain

More Related Videos

Modeling Neonatal Intraventricular Hemorrhage Through Intraventricular Injection of Hemoglobin
07:57

Modeling Neonatal Intraventricular Hemorrhage Through Intraventricular Injection of Hemoglobin

Published on: August 25, 2022

3.1K
Early Pathological and Magnetic Resonance Detection of Cerebral Injury Using a Rat Model of Neonatal Hypoxic Ischemic Encephalopathy
05:52

Early Pathological and Magnetic Resonance Detection of Cerebral Injury Using a Rat Model of Neonatal Hypoxic Ischemic Encephalopathy

Published on: October 28, 2022

842

Related Experiment Videos

Last Updated: Jul 20, 2026

Modeling Encephalopathy of Prematurity Using Prenatal Hypoxia-ischemia with Intra-amniotic Lipopolysaccharide in Rats
07:36

Modeling Encephalopathy of Prematurity Using Prenatal Hypoxia-ischemia with Intra-amniotic Lipopolysaccharide in Rats

Published on: November 20, 2015

11.5K
Modeling Neonatal Intraventricular Hemorrhage Through Intraventricular Injection of Hemoglobin
07:57

Modeling Neonatal Intraventricular Hemorrhage Through Intraventricular Injection of Hemoglobin

Published on: August 25, 2022

3.1K
Early Pathological and Magnetic Resonance Detection of Cerebral Injury Using a Rat Model of Neonatal Hypoxic Ischemic Encephalopathy
05:52

Early Pathological and Magnetic Resonance Detection of Cerebral Injury Using a Rat Model of Neonatal Hypoxic Ischemic Encephalopathy

Published on: October 28, 2022

842

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Immunology

Background:

  • Neonatal intraventricular hemorrhage (IVH) is a significant complication in preterm infants.
  • The influence of developmental stage on the brain's response to IVH is not well understood.

Purpose of the Study:

  • To investigate how different developmental stages affect the brain's transcriptional response to IVH.
  • To identify cell-type-specific changes following IVH across postnatal development.

Main Methods:

  • Single-nucleus RNA sequencing was performed on rat brains 24 hours after induced IVH.
  • Experiments were conducted at two distinct postnatal developmental stages: postnatal day 2 (PND2) and postnatal day 5 (PND5).
  • Analysis included identification of cell populations, differential gene expression, and pathway analysis (ligand-receptor, regulon).

Main Results:

  • Brains at PND5 showed a significantly stronger immune and inflammatory response to IVH compared to PND2, with threefold more differentially expressed genes.
  • Microglia were the most affected cell type, exhibiting oxidative stress and polarization towards both pro- and anti-inflammatory states at PND5.
  • A shift in signaling pathways was observed, from reparative IGF2 and TGF-β at PND2 to proinflammatory Wnt signaling and activation of Runx1 and Stat5 at PND5.

Conclusions:

  • Developmental timing critically influences the neuroimmune response to neonatal intraventricular hemorrhage.
  • Specific signaling pathways and cellular responses differ based on the brain's developmental stage post-injury.
  • Findings suggest potential stage-specific therapeutic targets for mitigating IVH-related brain injury.