Related Experiment Video
Updated: Jul 5, 2026
Protocol for Recombinant RBD-based SARS Vaccines: Protein Preparation, Animal Vaccination and Neutralization Detection
Published on: May 2, 2011
Adenovirus Nanoparticles Displaying RBD Induce a Protective Immune Response Against BA.5 in Mice
Chuncong Mo1,2, Zhongfang Wang1,2, Donglan Liu2
1State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Health, National Clinical Research Center for Respiratory Disease, National Center of Respiratory Medicine, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, Guangdong Province, 510182, People's Republic of China.
Introduction:
Adenovirus (Ad) vectors demonstrated significant efficacy as vaccine vectors during the COVID-19 pandemic. Hexon is the major capsid protein, and multiple hypervariable regions (HVRs) have been used for displaying exogenous antigens and inducing a strong antibody responses.
Methods:
We utilized SpyCatcher/SpyTag technology to incorporate SpyTag into HVR2, 4, and 7 of the hexon of the bivalent vaccine strain rAd3/7, respectively, to construct recombinant Ad, rAd3/7-SpyTag. The receptor-binding domain (RBD) of the SARS-CoV-2 BA5.2 strain fused with SpyCatcher was expressed as SpyCatcher-RBD, Spycatcher-RBD and rAd3/7-SpyTag were incubated in vitro to prepare a novel nanoparticle vaccine candidate, rAd3/7-SpyRBD, against SARS-CoV-2. Characterize rAd3/7-SpyRBD using Western blot, ELISA, transmission electron microscopy (TEM), and particle size measurement, and verify its immunogenicity through mouse immunization.
Results:
We have successfully established a universal nanoparticle vaccine platform, rAd3/7-SpyTag, and the RBD protein was successfully displayed on the surface of rAd3/7-SpyTag. Compared with SpyCatcher-RBD, rAd3/7-SpyRBD can rapidly induce the production of antibodies and stronger immune responses. Both Spycatcher-RBD and rAd3/7-SpyRBD provide a protective immune response against BA.5 in mouse model mice and can be used as candidates for SARS-CoV-2 vaccine. We also found that rAd3/7-SpyRBD induced the production of neutralizing antibodies against Ad3 and Ad7, suggested that it could serve as an Ads vaccine candidate.
Conclusion:
We developed a universal nanoparticle vaccine platform and obtained a trivalent vaccine candidate rAd3/7-SpyRBD, against SARS-CoV-2, Ad3, and Ad7, and this is the first time to use SpyCatcher/SpyTag technology in a bivalent rAd3/7 vector for trivalent immunity.
More Related Videos
13:36Utilizing the Antigen Capsid-Incorporation Strategy for the Development of Adenovirus Serotype 5-Vectored Vaccine Approaches
Published on: May 6, 2015
08:07A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain
Published on: July 25, 2022
