Related Experiment Video

Updated: Sep 11, 2025

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

9.1K

NXF1 suppresses progression of endometrial cancer by interacting with the SRSF3 to regulate SP4 splicing

Nan Meng1,2, Lingjun Li1, Xueqin Yang2

  • 1Jingmen Central Hospital, Jingmen Central Hospital Affiliated to Jingchu University of Technology, HuBei 448000, China.

Iscience
|August 12, 2025
PubMed

Insights

Nuclear export factor 1 (NXF1) suppresses endometrial cancer (ECa) progression by regulating SP4 alternative splicing. This finding offers a potential new biomarker for ECa treatment and prognosis.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Advanced endometriosis treatments face challenges with chemotherapy resistance, recurrence, and metastasis.
  • The role of Nuclear Factor X-interacting protein 1 (NXF1) in endometrial cancer (ECa) progression requires further investigation.

Purpose of the Study:

  • To investigate the function and molecular mechanisms of NXF1 in endometrial cancer (ECa) progression.
  • To determine if NXF1 can serve as a prognostic biomarker for ECa.

Main Methods:

  • Analysis of clinical endometrial cancer specimens.
  • In vitro and in vivo experiments using mouse models and molecular biology assays.
  • Investigation of NXF1's interaction with SRSF3 and SP4 splicing.

Main Results:

  • NXF1 expression inversely correlated with histological grade and patient prognosis.
  • NXF1 inhibited ECa cell proliferation, colony formation, migration, and invasion in vitro.
  • NXF1 suppressed tumor growth and metastasis in vivo by promoting the short SP4 isoform (S-SP4) over the long SP4 isoform (L-SP4) via an SRSF3-mediated mechanism.

Conclusions:

  • NXF1 acts as a tumor suppressor in endometrial cancer (ECa).
  • NXF1 inhibits ECa tumorigenicity and progression through an SRSF3-mediated SP4 alternative splicing mechanism.
  • NXF1 holds potential as a novel prognostic biomarker for clinical intervention in ECa.
Keywords:
CancerCell biologyMolecular biology

More Related Videos

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells06:48

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

Published on: June 16, 2022

2.1K
A Reporter Based Cellular Assay for Monitoring Splicing Efficiency08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

2.9K

Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
57.0K
Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
10.8K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.1K
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
21.7K
Transcriptional Regulation: Riboswitches01:23

Transcriptional Regulation: Riboswitches

Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
114
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
7.2K