LILRA5+ macrophages drive early oxidative stress surge in sepsis: a single-cell transcriptomic landscape with therapeutic implications
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View abstract on PubMed
Summary
This summary is machine-generated.Oxidative stress peaks in early sepsis, with macrophages showing the highest activity. The gene LILRA5, highly expressed in these macrophages, is a potential target for sepsis therapy.
Area Of Science
- Immunology
- Molecular Biology
- Biochemistry
Background
- Sepsis involves oxidative stress (OS), which influences adaptive responses.
- OS-related biomarkers are crucial for improving sepsis diagnosis and treatment.
Purpose Of The Study
- Identify immune cells with high oxidative activity in sepsis.
- Determine the role of oxidative stress in sepsis pathogenesis.
- Discover novel biomarkers for sepsis diagnosis and therapy.
Main Methods
- Single-cell datasets and OS gene sets were analyzed.
- High-dimensional weighted gene co-expression network analysis (hdWGCNA) and machine learning identified hub genes.
- Gene expression was validated using qRT-PCR and Western blotting.
- Reactive oxygen species (ROS) levels were measured after gene silencing.
Main Results
- Macrophages exhibit the highest OS in early sepsis.
- LILRA5, MGST1, PLBD1, and S100A9 were identified as upregulated hub genes.
- LILRA5 expression is highest in macrophages with the strongest OS, particularly in early sepsis.
- Silencing LILRA5 reduced ROS levels in THP-1 cells.
Conclusions
- This study maps OS dynamics in sepsis, highlighting LILRA5+ macrophages in early sepsis as having the highest OS.
- LILRA5 is a promising target for modulating macrophage-mediated OS in sepsis.
- Findings support LILRA5 as a potential biomarker and therapeutic target for sepsis.
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