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Author Spotlight: Integrated Multi-Omics Analysis for Unveiling Multicellular Immune Signatures in Clinical Heart Attack Cohorts
Published on: September 20, 2024
Exploring the biological basis for the identification of different syndromes in ischemic heart failure based on joint
Yilin Zhang1,2, Jingjing Wei1, Lijie Qiao1
1Department of Cardiovascular Disease, The First Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou, Henan, China.
Insights
This study reveals key biomarkers and pathways for three traditional Chinese medicine syndromes of ischemic heart disease (IHD) using multi-omics data. Findings link energy metabolism, hormonal regulation, and immune responses to IHD pathogenesis.
Area of Science:
- Integrative omics research
- Biomedical science
- Traditional Chinese Medicine (TCM) syndrome differentiation
Background:
- Ischemic heart disease (IHD) is a significant chronic illness.
- Qi deficiency and blood stasis syndrome (QDBS), Yang deficiency with blood stasis syndrome (YDBS), and Yang deficiency and blood stasis with fluid retention syndrome (YDBSFR) are core TCM syndromes of IHD.
- Understanding the biological underpinnings of these IHD syndromes is crucial.
Purpose of the Study:
- To explore the biological basis of QDBS, YDBS, and YDBSFR in IHD.
- To identify key biomarkers and enriched pathways associated with these IHD syndromes.
- To integrate multi-omics data for a comprehensive understanding of IHD pathogenesis.
Main Methods:
- Analysis and integration of transcriptomic, proteomic, and metabolomic data from IHD patients and healthy individuals.
- Construction of a network linking "Disease-Syndromes-Clinical phenotypes-Biomarkers-Pathways".
- External validation of identified differential genes and proteins.
Main Results:
- QDBS biomarkers (e.g., SDHD, IL10, Valeric acid) enriched in energy metabolism and immune pathways.
- YDBS biomarkers (e.g., TSHR, APOA2, 3-Hydroxybutyrate) linked to hormonal and lipid metabolism.
- YDBSFR biomarkers (e.g., CNGB1, C3, Oxamic acid) associated with hormone regulation, signal transduction, and energy metabolism.
- Platelet activation identified as a common factor in IHD progression.
Conclusions:
- This study provides a modern biomedical perspective on TCM syndromes of IHD.
- Identified biomarkers and pathways offer insights into the biological mechanisms of IHD.
- Findings support objective research in TCM syndrome differentiation for IHD.
Background:
IHF is a major chronic disease that seriously threatens human health. Qi deficiency and blood stasis syndrome (QDBS), Yang deficiency with blood stasis syndrome (YDBS) and Yang deficiency and blood stasis with fluid retention syndrome (YDBSFR) are the basic syndromes of IHF in Chinese medicine. This study aims to explore the biological basis of the three IHF syndromes through integrated multi-omics research.
Methods:
We analyzed and integrated transcriptomic, proteomic, and targeted metabolomic data from IHF patients and healthy persons to obtain the key biomarkers and enriched pathways of QDBS, YDBS and YDBSFR(Registration No.: ChiCTR2200058314). These biomarkers were combined with clinical indicators to construct the "Disease-Syndromes-Clinical phenotypes-Biomarkers-Pathways" network, and the obtained differential genes and proteins were externally validated.
Results:
The potential biomarkers for QDBS included SDHD, IL10, ACTG1, VWF, MDH2, COX5A, Valeric acid, Succinic Acid and L-Histidine, which were predominantly enriched in TCA cycle, oxidative phosphorylation, platelet activation, and neutrophil extracellular trap formation pathways, demonstrating associations with energy metabolism, coagulation system, and immune-inflammatory responses.YDBS potential biomarkers included TSHR, PRKG1, ATP1A2, GNAI2, APOA2, PLTP, 3-Hydroxybutyrate, Hexadecanoic acid and Palmitelaidic acid, and the combined pathways were mainly enriched in thyroid hormone synthesis, regulation of lipolysis in adipocytes, cholesterol metabolism and PPAR signaling pathways, correlating with hormonal regulation and lipid metabolism. The potential biomarkers of YDBSFR were CNGB1, KCNMA1, PIK3R2, HSPA8, C3, FH, Oxamic acid, N-Acetyl-L-alanine, 4-Hydroxyhippuric acid, and the combined pathways were mainly enriched in aldosterone-regulated sodium reabsorption, cGMP-PKG signaling pathway, neutrophil extracellular trap formation and TCA cycle signaling pathways, which are related to hormone regulation, signal transduction, immune-inflammatory response and energy metabolism. Platelet activation was involved in the whole process of IHF. External validation demonstrated the above core targets.
Conclusion:
This study investigated the biological basis of QDBS, YDBS and YDBSFR in IHF from a modern biomedical perspective, providing references for the objective research of TCM syndrome differentiation.
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