Simple Passive Environmental DNA Samplers for Marine Biomonitoring in Resource-Limited Programs
Patrick K Nichols1, Cécile M Vimond1, Peter B Marko1
1School of Life Sciences, University of Hawai'i at Mānoa, Honolulu, Hawai'i.
Current Protocols
|August 12, 2025
Summary
Passive environmental DNA samplers (PEDS) offer a simplified, low-cost method for aquatic biodiversity assessment. This approach enables easy eDNA capture without complex equipment, making it scalable for various environments.
Area of Science:
- Marine Biology
- Environmental Science
- Molecular Ecology
Background:
- Environmental DNA (eDNA) analysis is crucial for aquatic biodiversity assessment.
- Conventional eDNA methods often involve complex logistics and specialized equipment.
- There is a need for simplified, cost-effective eDNA sampling techniques.
Purpose of the Study:
- To introduce Passive Environmental DNA Samplers (PEDS) as a simplified alternative to active filtration.
- To provide a detailed protocol for PEDS construction, deployment, and DNA extraction.
- To demonstrate the efficacy of PEDS in detecting target species in marine environments.
Main Methods:
- PEDS utilize cotton membranes for passive eDNA capture, requiring no pumps or filtration systems.
- Protocols cover PEDS assembly, deployment (stationary or roving), retrieval, and sample processing.
- DNA extraction employs a modified Qiagen DNeasy Blood & Tissue protocol.
Main Results:
- PEDS allow for rapid deployment and retrieval with short field exposures (approx. 15 minutes).
- The method was successfully validated in the Papahānaumokuākea Marine National Monument.
- PEDS facilitated the detection of the nuisance alga Chondria tumulosa.
Conclusions:
- PEDS offer a cost-effective, scalable, and user-friendly approach for eDNA-based biodiversity monitoring.
- This protocol simplifies eDNA sampling, broadening its accessibility for researchers.
- PEDS are suitable for diverse aquatic environments, including marine ecosystems.
