Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Visual acuity in controls and patients measured with Maxwellian view and a 3 mm pupil: Examining potential effects of inherent and induced aberrations.

PloS one·2026

Same author

Single wavelength, dual temporal offset module for AOSLO systems.

Biomedical optics express·2026

Same author

Registration-Based Analysis of the Three-Dimensional Shape of the Retinal Nerve Fiber Layer for Detection of Glaucomatous Defects.

Translational vision science & technology·2026

Same author

Food Security Impacts Health of Children With Orofacial Clefts: An 8-Year Review of the Global Burden of Disease Study.

The Cleft palate-craniofacial journal : official publication of the American Cleft Palate-Craniofacial Association·2026

Same author

Targeted perimetry for mapping the shape of glaucomatous arcuate scotomata.

Ophthalmic & physiological optics : the journal of the British College of Ophthalmic Opticians (Optometrists)·2025

Same author

Longitudinal High-Resolution Imaging of Retinal Sequelae of a Choroidal Nevus.

Diagnostics (Basel, Switzerland)·2025

Same journal

Multifunctional reconfigurable terahertz metasurface based on vanadium dioxide phase transition: achieving broadband absorption and efficient polarization conversion.

Applied optics·2026

Same journal

High-Q-factor electromagnetically induced transparency utilizing quasi-bound states in the continuum in an all-dielectric terahertz metasurface.

Applied optics·2026

Same journal

Automated stitching interferometry for high-precision metrology of X-ray mirrors.

Applied optics·2026

Same journal

Experimental demonstration of an approach to designing a metal-dielectric DBR resonant cavity structure.

Applied optics·2026

Same journal

High-precision wavefront reconstruction from a single-shot interferogram using a physics-driven hybrid feature calibration network.

Applied optics·2026

Same journal

Ultra-high-Q Fano resonance based on coupled topological corner states in Kagome photonic crystals.

Applied optics·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Sep 11, 2025

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy Conpokal on Live Cells

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy Conpokal on Live Cells

Published on: August 11, 2020

Maximizing light efficiency in high-speed confocal line-scanning imagers.

Alessandra Carmichael-Martins, Thomas J Gast, Marcelina Sobczak

|August 12, 2025

Summary

This summary is machine-generated.

This study introduces an efficient optical design for high-speed en face imaging of the human eye. The novel asymmetric scanning system maximizes light collection for improved signal-to-noise ratio in ophthalmoscopy.

More Related Videos

Imaging Subcellular Structures in the Living Zebrafish Embryo

Imaging Subcellular Structures in the Living Zebrafish Embryo

Published on: April 2, 2016

Video-rate Scanning Confocal Microscopy and Microendoscopy

Video-rate Scanning Confocal Microscopy and Microendoscopy

Published on: October 20, 2011

Related Experiment Videos

Last Updated: Sep 11, 2025

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy Conpokal on Live Cells

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy Conpokal on Live Cells

Published on: August 11, 2020

Imaging Subcellular Structures in the Living Zebrafish Embryo

Imaging Subcellular Structures in the Living Zebrafish Embryo

Published on: April 2, 2016

Video-rate Scanning Confocal Microscopy and Microendoscopy

Video-rate Scanning Confocal Microscopy and Microendoscopy

Published on: October 20, 2011

Area of Science:

Ophthalmic imaging
Optical engineering
Biomedical optics

Background:

High-speed and efficient light capture are crucial for imaging the living human eye.
Minimizing light exposure while maximizing signal-to-noise ratio is a key challenge in ophthalmoscopy.

Purpose of the Study:

To report the optical design of a high-speed en face imaging approach for line-scanning laser ophthalmoscopy.
To achieve extremely high optical efficiency in ophthalmic imaging systems.

Main Methods:

Developed an asymmetric scanning system utilizing slit pupil scanning and full pupil light collection.
Engineered the system to project nearly all source light onto the target.
Designed for maximal collection of light returning from the target onto the detector.

Main Results:

The asymmetric system achieves nearly diffraction-limited performance.
Projected almost all light from the source onto the target.
Collected 98% of the light returning from the target onto the detector, significantly outperforming traditional systems.

Conclusions:

The novel asymmetric optical design offers superior light efficiency for high-speed en face ophthalmoscopy.
This approach enhances signal-to-noise ratio and minimizes light exposure compared to conventional methods.
The design provides a significant advancement for ocular imaging technologies.