Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

5.3K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
5.3K
Endoscopic Procedures III: Video Capsule Endoscopy01:28

Endoscopic Procedures III: Video Capsule Endoscopy

331
Capsule endoscopy, or wireless or video capsule endoscopy, is a diagnostic procedure for examining the entire gastrointestinal tract. Patients swallow a capsule about the size of a vitamin tablet. The capsule is equipped with a transmitter, a battery, an LED light source, and a color video camera to capture images throughout the gastrointestinal tract. This procedure is particularly useful for diagnosing conditions such as Crohn's disease, ulcerative colitis, tumors, polyps, ulcers,...
331
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.6K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tripeptide and hexapeptide topical as adjunct to nonablative fractional resurfacing for photodamage: A randomized split-face trial.

Journal of cosmetic dermatology·2020
Same author

Rare case of a basal cell carcinoma with intravascular invasion.

International journal of women's dermatology·2020
Same author

The Complete Genome Sequence of a Bacterial Strain with High Alkalic Xylanase Activity Isolated from the Sludge Near a Papermill.

Current microbiology·2020
Same author

Assessment of treatment tolerance and parental perspective of outpatient pulsed-dye laser treatment for port wine birthmark without general anesthesia in infants and toddlers.

Journal of the American Academy of Dermatology·2020
Same author

Association Between Non-high-density Lipoprotein Cholesterol and 3-Month Prognosis in Patients With Spontaneous Intracerebral Hemorrhage.

Frontiers in neurology·2020
Same author

The immune response after noise damage in the cochlea is characterized by a heterogeneous mix of adaptive and innate immune cells.

Scientific reports·2020

Related Experiment Video

Updated: Sep 11, 2025

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
14:09

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

7.0K

Athermalization and large depth of field in endoscopes based on wavefront coding.

Kun Liu, Lijiao Wang, Zhiying Yang

    Applied Optics
    |August 12, 2025
    PubMed
    Summary

    Wavefront coding (WFC) technology enables industrial endoscopes to achieve extended depth of field (DOF) and a wider operational temperature range. This study validates WFC

    More Related Videos

    Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
    10:07

    Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

    Published on: April 9, 2014

    10.1K
    Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
    12:54

    Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

    Published on: October 2, 2021

    3.4K

    Related Experiment Videos

    Last Updated: Sep 11, 2025

    High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
    14:09

    High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

    Published on: November 16, 2019

    7.0K
    Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
    10:07

    Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

    Published on: April 9, 2014

    10.1K
    Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
    12:54

    Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

    Published on: October 2, 2021

    3.4K

    Area of Science:

    • Optical Engineering
    • Imaging Technology
    • Materials Science

    Background:

    • Traditional industrial endoscopes face limitations in depth of field (DOF) and operational temperature range.
    • Existing technologies often require trade-offs between DOF extension and thermal stability.
    • The need for robust imaging solutions in extreme temperature environments is critical for industrial applications.

    Purpose of the Study:

    • To theoretically validate the feasibility of wavefront coding (WFC) for simultaneous DOF extension and athermalization in industrial endoscopes.
    • To design and optimize an industrial endoscope incorporating an expanded quintic phase mask for enhanced performance.
    • To investigate the impact of WFC on image quality and recoverability across varying object distances and temperatures.

    Main Methods:

    • Theoretical analysis of wavefront coding (WFC) principles applied to optical system design.
    • Design and optimization of an industrial endoscope (F-number 8, focal length 7 mm, length 871 mm, FOV 52°).
    • Implementation of an expanded quintic phase mask for DOF extension and athermalization.
    • Utilized dynamic data exchange for modulation transfer function consistency and image recoverability.
    • Deblurred intermediate images using Wiener filtering with specific point spread functions.

    Main Results:

    • The depth of focus was successfully extended from 0.075 mm to 0.25 mm.
    • The depth of field (DOF) was significantly extended from 12.18 mm to infinity.
    • The system demonstrated an operational temperature range from 20°C to 1200°C.
    • Optimized phase mask ensured consistent image quality across diverse conditions.

    Conclusions:

    • Wavefront coding (WFC) technology is feasible for simultaneously extending the depth of field (DOF) and operational temperature range of industrial endoscopes.
    • The designed endoscope with an expanded quintic phase mask achieves significant improvements in DOF and thermal stability.
    • This WFC approach offers a promising solution for high-performance industrial imaging in extreme environments.