Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.5K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.5K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

294
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
294
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

5.3K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
5.3K
Computed Tomography01:10

Computed Tomography

6.2K
Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...
6.2K
2D NMR: Homonuclear Correlation Spectroscopy (COSY)01:06

2D NMR: Homonuclear Correlation Spectroscopy (COSY)

1.3K
Homonuclear correlation spectroscopy, or COSY, is a 2-dimensional NMR technique that provides information about coupled protons. Typically, the geminal and vicinal coupling are observed. For example, consider the COSY spectrum of ethyl acetate, where its 1D proton NMR spectrum is plotted along the vertical and horizontal axes with their corresponding chemical shift scale. Three spots on the diagonal corresponding to the three peaks in the 1D proton spectrum are called diagonal peaks. The COSY...
1.3K
2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

292
Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
292

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Advances in Digital Holography, 3-D Imaging, and Optical Information Processing: introduction.

Biomedical optics express·2026
Same author

3D-DeepZern: a deep convolutional neural network for tomographic reconstruction based on Zernike polynomials.

Applied optics·2026
Same author

Deep learning caustic image generation.

Applied optics·2026
Same author

Advances in digital holography, 3-D imaging, and optical information processing: introduction.

Applied optics·2026
Same author

Advances in digital holography, 3-D imaging, and optical information processing: introduction.

Journal of the Optical Society of America. A, Optics, image science, and vision·2026
Same author

Digital Holographic Microscopy for Phenotypic Profiling of Adherent Cells.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Multifunctional reconfigurable terahertz metasurface based on vanadium dioxide phase transition: achieving broadband absorption and efficient polarization conversion.

Applied optics·2026
Same journal

High-Q-factor electromagnetically induced transparency utilizing quasi-bound states in the continuum in an all-dielectric terahertz metasurface.

Applied optics·2026
Same journal

Automated stitching interferometry for high-precision metrology of X-ray mirrors.

Applied optics·2026
Same journal

Experimental demonstration of an approach to designing a metal-dielectric DBR resonant cavity structure.

Applied optics·2026
Same journal

High-precision wavefront reconstruction from a single-shot interferogram using a physics-driven hybrid feature calibration network.

Applied optics·2026
Same journal

Ultra-high-Q Fano resonance based on coupled topological corner states in Kagome photonic crystals.

Applied optics·2026
See all related articles

Related Experiment Video

Updated: Sep 11, 2025

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects
10:16

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

Published on: February 8, 2014

12.4K

Fast deep coherence holography for 3D object reconstruction.

Quang Trieu, George Nehmetallah

    Applied Optics
    |August 12, 2025
    PubMed
    Summary
    This summary is machine-generated.

    We introduce fast deep coherence holography (FDCH), a novel deep learning method for reconstructing 3D objects from holographic images. This technique significantly reduces reconstruction time and improves accuracy, requiring only two images from any camera position.

    More Related Videos

    Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
    10:28

    Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

    Published on: July 5, 2016

    10.4K
    Recording Ultra-Realistic Full-Color Analog Holograms for Use in a Moving Hologram Display
    09:04

    Recording Ultra-Realistic Full-Color Analog Holograms for Use in a Moving Hologram Display

    Published on: January 14, 2020

    9.8K

    Related Experiment Videos

    Last Updated: Sep 11, 2025

    Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects
    10:16

    Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

    Published on: February 8, 2014

    12.4K
    Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
    10:28

    Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

    Published on: July 5, 2016

    10.4K
    Recording Ultra-Realistic Full-Color Analog Holograms for Use in a Moving Hologram Display
    09:04

    Recording Ultra-Realistic Full-Color Analog Holograms for Use in a Moving Hologram Display

    Published on: January 14, 2020

    9.8K

    Area of Science:

    • Optics and Photonics
    • Computational Imaging
    • Deep Learning Applications

    Background:

    • Traditional phase-shift coherence holography reconstructs complex objects but is susceptible to noise.
    • Previous deep coherence holography (DCH) required precise camera positioning and multiple images, increasing experimental complexity and cost.

    Purpose of the Study:

    • To develop a fast, position-independent reconstruction method for coherence holography using deep learning.
    • To overcome the limitations of existing methods in terms of speed, accuracy, and experimental setup complexity.

    Main Methods:

    • Proposed a novel deep learning (DL) method named fast deep coherence holography (FDCH).
    • Trained a network to reconstruct the complex object field at the camera's position, enabling 3D object reconstruction via angular spectrum propagation.
    • FDCH requires only two images captured at a random camera position.

    Main Results:

    • FDCH achieves position-independent 3D object reconstruction.
    • Significantly reduces total reconstruction time compared to analytical methods and DCH.
    • Yields higher accuracy in reconstruction compared to previous methods.

    Conclusions:

    • FDCH offers a faster and more accurate method for 3D object reconstruction in coherence holography.
    • The position-independent nature and minimal image requirement simplify experimental setups.
    • This deep learning approach represents a significant advancement in holographic imaging.