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Published on: November 1, 2019
Evaluation of the Variability in Selenoprotein Genes in Hepatocellular Carcinoma Patients
Andressa de Freitas Alves1, Vanessa Dido Baldissera2, Tatiane Jacobsen da Rocha2,3
1Biosciences Graduate Program, Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA), Porto Alegre, Rio Grande do Sul, Brazil.
Abstract:
Hepatocarcinogenesis is associated with various factors, including oxidative stress. Alterations in selenoprotein genes could impair redox balance and influence cancer development. In this study, we aimed to evaluate the association of single nucleotide variants (SNVs) from selenoproteins with hepatocellular carcinoma risk. The case and healthy groups were genotyped using quantitative polymerase chain reaction (qPCR), and the analyzed SNVs were rs1050450 and rs3448 GPX1, rs713041 GPX4, rs5845 and rs5859 SEP15, and rs7579 and rs3877899 SELENOP. Significant differences in genotype frequencies were observed between the case and healthy groups (p < 0.05) for all studied SNVs, except for GPX1 rs3448. Furthermore, G/G rs1050450 GPX1 (OR = 1.975; 95% CI 1.075-3.628; p = 0.028) and homozygous C/C rs7579 SELENOP (OR = 3.088; 95% CI 1.667-5.722; p < 0.001) were associated with an increased risk of hepatocellular carcinoma. Comparisons with 1000 Genomes Project data revealed genotype frequencies similar to those of European descendants. These results could suggest a role of genetic alterations of selenoproteins in hepatocellular carcinoma risk.
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