Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Structure and Function of Platelets01:18

Structure and Function of Platelets

1.5K
The cell fragments known as platelets are disc-shaped, with an average diameter of about 3 μm and a thickness of roughly 1 μm. They play a crucial role in the body's vascular clotting system, which also involves plasma proteins, blood cells, and blood vessel tissues.
Platelets are continually replenished, circulating in the bloodstream for 9-12 days before being removed by phagocytes, primarily in the spleen. A microliter of circulating blood contains between 150,000 and 450,000...
1.5K
Formation of the Platelet Plug01:22

Formation of the Platelet Plug

7.1K
The platelet phase, the second stage of hemostasis, commences around 15-20 seconds after an injury. It follows and overlaps with the vascular phase, during which blood vessels constrict to minimize blood loss.
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...
7.1K
Inflammation01:38

Inflammation

55.1K
Overview
55.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Interleukin-17A mediates cardiorenal injury in oxalate nephropathy.

Cardiovascular research·2026
Same author

LASP1, a Novel Protein in Spermatozoa and Acrosome Reaction.

Molecular reproduction and development·2026
Same author

NK cells promote cardiac cell death and regulate myelopoiesis in myocardial infarction.

Nature communications·2026
Same author

Blocking microglial reactivity via purinergic receptors prevents subacute cognitive deficits after TIA.

EMBO molecular medicine·2026
Same author

European Research Council-funded grant: drivers of thrombo-inflammation.

European heart journal·2026
Same author

Thrombo-inflammatory endothelial signatures in <i>JAK2</i> -mutated myeloproliferative neoplasms.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Sep 11, 2025

Real-time Imaging of Heterotypic Platelet-neutrophil Interactions on the Activated Endothelium During Vascular Inflammation and Thrombus Formation in Live Mice
11:18

Real-time Imaging of Heterotypic Platelet-neutrophil Interactions on the Activated Endothelium During Vascular Inflammation and Thrombus Formation in Live Mice

Published on: April 2, 2013

15.8K

Platelets drive macrophage inflammatory activation in vitro.

Anna Rizakou1,2, Annabelle Rosa2, Lukas Johannes Weiss2,3

  • 1Rudolf Virchow Center for Integrative and Translational Bioimaging, Julius-Maximilians-Universität Würzburg, Josef Schneider Str. 2, 97080 Würzburg, Germany.

Journal of Leukocyte Biology
|August 12, 2025
PubMed
Summary

Platelets interact with macrophages, promoting a proinflammatory response in heart injury models. This platelet-macrophage interaction, largely contact-dependent, influences macrophage activation and gene expression in the ischemic heart.

Keywords:
inflammationmacrophagesmyocardial infarctionplatelets

More Related Videos

Laminar Flow-based Assays to Investigate Leukocyte Recruitment on Cultured Vascular Cells and Adherent Platelets
08:50

Laminar Flow-based Assays to Investigate Leukocyte Recruitment on Cultured Vascular Cells and Adherent Platelets

Published on: April 9, 2018

7.7K
Procoagulant Platelet Characterization by Measuring Phosphatidylserine Exposure and Microvesicle Release from Human Purified Platelets
05:49

Procoagulant Platelet Characterization by Measuring Phosphatidylserine Exposure and Microvesicle Release from Human Purified Platelets

Published on: November 29, 2024

718

Related Experiment Videos

Last Updated: Sep 11, 2025

Real-time Imaging of Heterotypic Platelet-neutrophil Interactions on the Activated Endothelium During Vascular Inflammation and Thrombus Formation in Live Mice
11:18

Real-time Imaging of Heterotypic Platelet-neutrophil Interactions on the Activated Endothelium During Vascular Inflammation and Thrombus Formation in Live Mice

Published on: April 2, 2013

15.8K
Laminar Flow-based Assays to Investigate Leukocyte Recruitment on Cultured Vascular Cells and Adherent Platelets
08:50

Laminar Flow-based Assays to Investigate Leukocyte Recruitment on Cultured Vascular Cells and Adherent Platelets

Published on: April 9, 2018

7.7K
Procoagulant Platelet Characterization by Measuring Phosphatidylserine Exposure and Microvesicle Release from Human Purified Platelets
05:49

Procoagulant Platelet Characterization by Measuring Phosphatidylserine Exposure and Microvesicle Release from Human Purified Platelets

Published on: November 29, 2024

718

Area of Science:

  • Immunology
  • Cardiovascular Biology
  • Cell Biology

Background:

  • Macrophages play a dual role in tissue healing, mediating repair but also damage and fibrosis.
  • Platelets are key inflammatory mediators that interact with macrophages, potentially modulating their phenotype.
  • The precise impact of platelets on macrophage gene expression, particularly in sterile injury contexts, remains incompletely understood.

Purpose of the Study:

  • To investigate the in vitro effects of platelets on macrophage activation and gene expression.
  • To explore platelet-macrophage interactions in a model of sterile tissue injury, specifically experimental myocardial infarction (MI).
  • To determine the contact dependency of platelet-induced macrophage proinflammatory polarization.

Main Methods:

  • Utilized bulk RNA-sequencing to analyze gene expression changes in mouse bone marrow-derived macrophages co-cultured with activated or resting platelets.
  • Employed fluorescence microscopy to visualize direct platelet-macrophage interactions in an experimental myocardial infarction mouse model.
  • Used transwell assays to assess the contact dependency of platelet-macrophage communication.

Main Results:

  • Co-culture with activated platelets induced widespread proinflammatory gene upregulation in macrophages, including genes for inflammation (Il1b, Trem1, Tlr2, Cd14), angiogenesis (Vegfa), and hypoxia response (Hif1a).
  • Resting platelets also elicited inflammatory gene expression, though to a lesser extent than activated platelets.
  • Platelets and platelet-derived CXCL4 had minimal impact on profibrotic gene expression in macrophages.
  • Proinflammatory effects of platelets on macrophage transcriptomes were largely dependent on direct cell contact.

Conclusions:

  • Platelets interact directly with macrophages in the ischemic heart.
  • Platelets, particularly activated ones, polarize macrophages towards a proinflammatory phenotype in vitro.
  • These findings suggest a significant role for platelet-macrophage interactions in driving cardiac macrophage inflammatory activation post-myocardial infarction.