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Does determination of polyamines in body fluids require heating with hydrochloric acid?
Methods and Findings in Experimental and Clinical Pharmacology
|October 1, 1985
Summary
Hydrolysis of plasma and urine using concentrated hydrochloric acid does not improve the recovery of spermidine. This method is ineffective for increasing spermidine yield from biological samples.
Area of Science:
- Biochemistry
- Analytical Chemistry
Background:
- Spermidine is a polyamine found in various body fluids.
- Accurate quantification of spermidine is important for understanding its biological roles.
Purpose of the Study:
- To evaluate the effectiveness of hydrochloric acid hydrolysis for enhancing spermidine recovery from plasma and urine.
Main Methods:
- Hydrolysis of plasma and urine samples using concentrated hydrochloric acid and heat.
- Analysis of spermidine recovery before and after hydrolysis.
Main Results:
- Hydrolysis with concentrated hydrochloric acid did not lead to an increased recovery of spermidine.
- The applied method did not improve the yield of spermidine from the biological matrices.
Conclusions:
- Hydrolysis by heating with concentrated hydrochloric acid is not a suitable method for increasing spermidine recovery from plasma and urine.
- Alternative methods should be explored for optimal spermidine extraction and quantification.