Optimizing T cell transduction: a novel transduction device for efficient and scalable gene delivery

Area of Science:

• Biotechnology
• Cell Therapy Manufacturing
• Gene Delivery

Background:

• Viral transduction is essential for genetically modified T cell production in immunotherapies.
• Conventional methods face challenges including low efficiency, high vector use, and scalability issues.

Purpose of the Study:

• To introduce an innovative platform, the Transduction Boosting Device (TransB), for efficient and scalable gene delivery.
• To overcome limitations of current T cell transduction techniques.

Main Methods:

• The Transduction Boosting Device (TransB) is an automated, closed-system platform designed to enhance cell-virus proximity.
• Evaluated TransB's impact on donor T cell transduction using Lenti-GFP vectors compared to standard 24-well plates.

Main Results:

• TransB reduced processing time by up to 1-fold and viral vector consumption by 3-fold, with a 0.7-fold increase in transduction efficiency.
• Achieved an average 0.5-fold improvement in transduction efficiency across multiple donors, maintaining cell viability and phenotype.
• Demonstrated consistent performance across varying input cell numbers, confirming scalability.

Conclusions:

• TransB significantly shortens transduction time, lowers costs, and boosts efficiency in T cell therapy manufacturing.
• The platform shows potential as a robust solution to enhance T cell therapy production and address manufacturing challenges.