Targeted delivery of diverse biomolecules with engineered bacterial nanosyringes
Joseph Kreitz1,2,3,4,5,6,7, Victoria Yang1,2,3,4,5,6,7, Mirco J Friedrich1,2,3,4,5,6,7
1Howard Hughes Medical Institute, Cambridge, MA, USA.
Nature Biotechnology
|August 12, 2025
Abstract
None:
The Photorhabdus virulence cassette is a microbial nanosyringe that can be engineered to deliver protein cargos into human cells. Here we further modify this system to incorporate exogenous cargos and targeting moieties in vitro. We show that this method, termed SPEAR, enables loading of different types of cargo (including folded ribonucleoproteins and single-stranded DNA) and targeting of defined cell types both in vitro and in vivo.
