Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

14.3K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
14.3K
Light Acquisition02:16

Light Acquisition

8.6K
In order to produce glucose, plants need to capture sufficient light energy. Many modern plants have evolved leaves specialized for light acquisition. Leaves can be only millimeters in width or tens of meters wide, depending on the environment. Due to competition for sunlight, evolution has driven the evolution of increasingly larger leaves and taller plants, to avoid shading by their neighbors with contaminant elaboration of root architecture and mechanisms to transport water and nutrients.
8.6K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.6K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.6K
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

6.6K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
6.6K
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

5.3K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
5.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Electrochemical in-biosensing computing.

National science review·2026
Same author

Intimate Interaction Between Nucleic Acid and Conjugated Polymers in Organic Electrochemical Transistors Enables Ultrasensitive Biomarker Detection.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

A bioinspired photoelectrochemical synapse for neurotransmitter-mediated in-sensor computing.

Biosensors & bioelectronics·2026
Same author

Deep learning for predicting lumbar segmental instability using neutral lateral lumbar radiographs: a retrospective study.

European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·2026
Same author

210 W, 8.4 mJ Yb:YAG picosecond bulk amplifier.

Optics express·2026
Same author

Ultrasound-Assisted Zwitterion Grafting on NiO<sub>x</sub> for Suppressing Self-Assembled Monolayer Migration in Perovskite Solar Cells.

Advanced materials (Deerfield Beach, Fla.)·2026
Same journal

Denoising algorithm of Φ-OTDR systems based on adaptive fractional wavelet transform denoising.

Optics express·2026
Same journal

Millisecond photon-to-photon latency and high-speed volumetric projection system for optogenetics.

Optics express·2026
Same journal

Polarization-encoded coaxial structured light for high-precision 3D surface profilometry.

Optics express·2026
Same journal

Discrete freeform optical design based on collaborative optimization of point cloud and local normals.

Optics express·2026
Same journal

Ultrafast ghost imaging with 25 GHz speckle switching and wavelength-division multiplexing.

Optics express·2026
Same journal

Atomic vapor cells fabricated by femtosecond laser welding of standard-optical-quality glass.

Optics express·2026
See all related articles

Related Experiment Video

Updated: Sep 11, 2025

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

25.4K

Single-shot structured light illumination based on multiscale total generalized variation.

Jiajun Song, Qinghui Zhang, Lei Lu

    Optics Express
    |August 13, 2025
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces a new single-shot 3D reconstruction method using multiscale total generalized variation. It significantly improves accuracy on textured surfaces by reducing spectral leakage and frequency aliasing.

    More Related Videos

    Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
    08:41

    Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

    Published on: August 16, 2012

    11.6K
    Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
    10:07

    Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

    Published on: April 9, 2014

    10.1K

    Related Experiment Videos

    Last Updated: Sep 11, 2025

    A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
    11:15

    A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

    Published on: May 30, 2016

    25.4K
    Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
    08:41

    Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

    Published on: August 16, 2012

    11.6K
    Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
    10:07

    Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

    Published on: April 9, 2014

    10.1K

    Area of Science:

    • Computer Vision
    • Optical Metrology

    Background:

    • Single-shot 3D reconstruction is vital for high-speed applications.
    • Fourier transform profilometry struggles with textured surfaces due to spectral leakage and aliasing.

    Purpose of the Study:

    • To develop a robust single-shot 3D reconstruction framework for complex surfaces.
    • To enhance accuracy by addressing limitations of conventional Fourier transform profilometry.

    Main Methods:

    • Proposed a framework using multiscale total generalized variation and variational optimization.
    • Integrated multi-frequency fringe modulation to generate dual-frequency heterodyne patterns.
    • Employed an adaptive orientation total generalized variation model for fringe component extraction.

    Main Results:

    • Achieved superior reconstruction quality on highly textured surfaces.
    • Demonstrated enhanced robustness compared to traditional methods.
    • Effectively suppressed spectral overlap using the dual-frequency heterodyne technique.

    Conclusions:

    • The proposed method offers a significant advancement in single-shot 3D reconstruction for challenging surfaces.
    • Multiscale total generalized variation effectively handles complex textures.
    • The framework provides a more accurate and robust solution for high-speed 3D imaging.