Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

6.6K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
6.6K
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

5.3K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
5.3K
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

9.5K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
9.5K
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.5K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.5K
Reflection of Waves01:07

Reflection of Waves

3.9K
When a wave travels from one medium to another, it gets reflected at the boundary of the second medium. A common example of this is when a person yells at a distance from a cliff and hears the echo of their voice. The sound waves (longitudinal waves) traveling in the air are reflected from the bounding cliff. Similarly, flipping one end of a string whose other end is tied to a wall causes a pulse (transverse wave) to travel through the string, which gets reflected upon reaching the wall. In...
3.9K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

14.3K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
14.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Clinical Analysis of Torque Teno Virus Infection after Hematopoietic Stem Cell Transplantation in Children].

Zhongguo shi yan xue ye xue za zhi·2026
Same author

Integration of eQTL and GEO Datasets to Identify Genes Associated with Breast Ductal Carcinoma In Situ.

Current issues in molecular biology·2025
Same author

New generation scheme of optical knots and links based on polarization of light field and its application.

Optics express·2025
Same author

Retraction Note: NR3C2 affects the proliferation and invasiveness of colon cancer cells through the Wnt/β-Catenin signaling pathway.

Journal of cancer research and clinical oncology·2025
Same author

[Manipulation treatment of lumbar disc herniation based on the model of muscles and bones assessment].

Zhongguo gu shang = China journal of orthopaedics and traumatology·2024
Same author

NR3C2 affects the proliferation and invasiveness of colon cancer cells through the Wnt/β-Catenin signaling pathway.

Journal of cancer research and clinical oncology·2024

Related Experiment Video

Updated: Sep 11, 2025

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
12:54

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

3.4K

Multi-reflection quantum-optical coherence tomography and experimental mimic with the classical optical field.

Qian Li, Yifan Sun, Ling-Jun Kong

    Optics Express
    |August 13, 2025
    PubMed
    Summary

    Multi-reflection quantum optical coherence tomography (MQOCT) enhances axial resolution by a factor of N, surpassing classical OCT. A classical analogy offers similar benefits without quantum light source fragility.

    More Related Videos

    Spectral and Angle-Resolved Magneto-Optical Characterization of Photonic Nanostructures
    08:01

    Spectral and Angle-Resolved Magneto-Optical Characterization of Photonic Nanostructures

    Published on: November 21, 2019

    7.2K
    Quantum State Engineering of Light with Continuous-wave Optical Parametric Oscillators
    09:23

    Quantum State Engineering of Light with Continuous-wave Optical Parametric Oscillators

    Published on: May 30, 2014

    14.6K

    Related Experiment Videos

    Last Updated: Sep 11, 2025

    Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
    12:54

    Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

    Published on: October 2, 2021

    3.4K
    Spectral and Angle-Resolved Magneto-Optical Characterization of Photonic Nanostructures
    08:01

    Spectral and Angle-Resolved Magneto-Optical Characterization of Photonic Nanostructures

    Published on: November 21, 2019

    7.2K
    Quantum State Engineering of Light with Continuous-wave Optical Parametric Oscillators
    09:23

    Quantum State Engineering of Light with Continuous-wave Optical Parametric Oscillators

    Published on: May 30, 2014

    14.6K

    Area of Science:

    • Quantum optics
    • Metrology
    • Biomedical imaging

    Background:

    • Quantum optical coherence tomography (QOCT) utilizes quantum interference for enhanced imaging.
    • Current QOCT research primarily focuses on two-photon systems, achieving twofold axial resolution improvement.
    • Classical optical coherence tomography (OCT) is a widely used imaging technique.

    Purpose of the Study:

    • To introduce a theoretical framework for multi-reflection quantum-optical coherence tomography (MQOCT).
    • To investigate the potential for enhanced axial resolution in QOCT using multiple reflections.
    • To develop and validate a classical optical analogy for MQOCT.

    Main Methods:

    • Theoretical framework development for MQOCT.
    • Incorporation of a multiple reflections strategy into the QOCT scheme.
    • Experimental validation of a classical optical analogy using P-beam correlated multi-mode broadband beams.

    Main Results:

    • MQOCT theoretically enhances axial resolution by a factor of N (number of reflections) compared to QOCT.
    • The proposed MQOCT scheme preserves the dispersion cancellation capability of QOCT.
    • The classical optical analogy demonstrates equivalent resolution enhancement and dispersion cancellation without quantum light source fragility.

    Conclusions:

    • MQOCT offers significant theoretical advantages in axial resolution for QOCT.
    • A robust classical optical analogy of MQOCT can be realized, overcoming quantum light source limitations.
    • This work paves the way for more advanced and practical QOCT-based imaging techniques.