Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

9.5K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
9.5K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.6K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.6K
Atomic Emission Spectroscopy: Interference01:30

Atomic Emission Spectroscopy: Interference

274
In atomic emission spectroscopy (AES), high-temperature atomizers excite a broad range of elements and molecules that generate complex emissions from sources such as oxides, hydroxides, and flame combustion products in the flame or plasma. Several strategies can be employed to minimize spectral interferences caused by overlapping emission lines or bands. These include increasing instrument resolution, choosing alternative emission lines, optimally placing the detector in low-background regions,...
274
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

14.3K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
14.3K
Atomic Absorption Spectroscopy: Interference01:25

Atomic Absorption Spectroscopy: Interference

1.1K
Interference leads to systematic error in atomic absorption (AA) measurements by enhancing or diminishing the analytical signal or the background. These interferences can be grouped into three main categories: spectral interference, chemical interference, and physical interference.
Spectral interference occurs when signals from other elements or molecules overlap with the analyte signal, falsely elevating or masking the analyte's absorbance. This interference can be corrected using Zeeman,...
1.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

How organizational support promotes teacher professional recognition: a perspective on teachers' autonomous learning and teaching abilities.

Frontiers in psychology·2026
Same author

Both chronological age and individual differences in aging are the two indispensable components for predicting biological age.

Computer methods in biomechanics and biomedical engineering·2026
Same author

Hmgcs1 regulates cholesterol synthesis and promotes nerve fiber repair after spinal cord injury.

iScience·2026
Same author

Integrated multi-omics analysis unveils microbiota-metabolite-host interactions and novel biomarkers for early diabetic kidney disease diagnosis.

Frontiers in immunology·2026
Same author

ZRMQ-22, a novel DYRK1A inhibitor, attenuates neuroinflammation and cognitive impairments in LPS-induced mice: a potential strategy for Alzheimer's disease.

RSC medicinal chemistry·2026
Same author

Novel β-Carboline Derivative ZLWH-67 Exerts Potent Anti-MRSA Activity through Multiple Mechanisms.

ACS infectious diseases·2026
Same journal

Denoising algorithm of Φ-OTDR systems based on adaptive fractional wavelet transform denoising.

Optics express·2026
Same journal

Millisecond photon-to-photon latency and high-speed volumetric projection system for optogenetics.

Optics express·2026
Same journal

Polarization-encoded coaxial structured light for high-precision 3D surface profilometry.

Optics express·2026
Same journal

Discrete freeform optical design based on collaborative optimization of point cloud and local normals.

Optics express·2026
Same journal

Ultrafast ghost imaging with 25 GHz speckle switching and wavelength-division multiplexing.

Optics express·2026
Same journal

Atomic vapor cells fabricated by femtosecond laser welding of standard-optical-quality glass.

Optics express·2026
See all related articles

Related Experiment Video

Updated: Sep 11, 2025

Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy FSM
19:16

Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy FSM

Published on: August 5, 2009

16.1K

Speckle suppression in dynamic structured light via single-element interference.

Mengyu Ren, Ji Cui, Si-Ao Cai

    Optics Express
    |August 13, 2025
    PubMed
    Summary
    This summary is machine-generated.

    This study presents a new dynamic stripe fluorescence method for 3D measurement. It effectively suppresses laser speckles, enabling accurate 3D reconstruction with high-quality stripe projection.

    More Related Videos

    A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
    11:15

    A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

    Published on: May 30, 2016

    25.4K
    Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator
    08:39

    Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

    Published on: January 28, 2019

    9.9K

    Related Experiment Videos

    Last Updated: Sep 11, 2025

    Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy FSM
    19:16

    Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy FSM

    Published on: August 5, 2009

    16.1K
    A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
    11:15

    A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

    Published on: May 30, 2016

    25.4K
    Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator
    08:39

    Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

    Published on: January 28, 2019

    9.9K

    Area of Science:

    • Optics and Photonics
    • Metrology
    • Computer Vision

    Background:

    • Structured light 3D measurement technology relies on projecting patterns to capture object depth and morphology.
    • Laser-based methods, while good for fine features, suffer from speckle noise interfering with phase detection.
    • Accurate 3D reconstruction is crucial in various scientific and industrial applications.

    Purpose of the Study:

    • To introduce a novel dynamic stripe fluorescence measurement method for 3D reconstruction.
    • To overcome the limitations of laser speckle interference in phase detection.
    • To achieve high-precision 3D measurements with improved stripe quality.

    Main Methods:

    • Developed a dynamic stripe fluorescence measurement technique utilizing single-element interference.
    • Employed fluorescence emission to suppress laser speckles.
    • Implemented a simple and easily structured system for stripe projection and phase digitization.

    Main Results:

    • Successfully suppressed laser speckles through fluorescence emission.
    • Achieved high-quality stripe projection on object surfaces.
    • Demonstrated accurate 3D reconstruction capabilities with the proposed method.

    Conclusions:

    • The dynamic stripe fluorescence method offers a robust solution for speckle-free 3D measurements.
    • This technique simplifies the 3D reconstruction process while maintaining high accuracy.
    • The approach is practical for applications requiring precise surface morphology capture.