Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Fungal Phylum Ascomycota01:28

Fungal Phylum Ascomycota

147
Phylum Ascomycota, a major division within the subkingdom Dikarya, comprises a diverse range of fungal species, including both unicellular yeasts and filamentous molds such as Aspergillus and Penicillium. These fungi thrive in a variety of habitats, from aquatic ecosystems to terrestrial environments, playing crucial ecological and economic roles.Morphology and ReproductionThe defining characteristic of Ascomycetes, commonly referred to as sac fungi, is the ascus—a sac-like structure that...
147
  1. Home
  3. Research Domains
  5. Chemical Sciences
  7. Inorganic Chemistry
  9. Inorganic Chemistry Not Elsewhere Classified
  11. Asperustins K-p, Chlorinated Austocystins From Aspergillus Ustus Nrrl 5856.
  1. Home
  3. Research Domains
  5. Chemical Sciences
  7. Inorganic Chemistry
  9. Inorganic Chemistry Not Elsewhere Classified
  11. Asperustins K-p, Chlorinated Austocystins From Aspergillus Ustus Nrrl 5856.

Related Experiment Video

Natural Product Discovery with LC-MS/MS Diagnostic Fragmentation Filtering: Application for Microcystin Analysis
07:18

Natural Product Discovery with LC-MS/MS Diagnostic Fragmentation Filtering: Application for Microcystin Analysis

Published on: May 31, 2019

9.0K

Asperustins K-P, Chlorinated Austocystins from Aspergillus ustus NRRL 5856.

Chun-Lun Qin1, Xiao-Kun Yu1, Yin-Hui Zhou1

  • 1Huazhong University of Science and Technology, Tongji Medical College, School of Pharmacy, Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, Wuhan 430030, People's Republic of China.

Organic Letters
|August 13, 2025

View abstract on PubMed

Summary
This summary is machine-generated.

Six new chlorinated austocystins, asperustins K-P, were discovered from Aspergillus ustus. These compounds exhibit potent immunosuppressive activities, offering potential for new therapeutic agents.

More Related Videos

Isolation of Culturable Yeasts and Molds from Soils to Investigate Fungal Population Structure
10:33

Isolation of Culturable Yeasts and Molds from Soils to Investigate Fungal Population Structure

Published on: May 27, 2022

5.8K
Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the α-amylase Inhibitor from Lablab purpureus L.
09:21

Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the α-amylase Inhibitor from Lablab purpureus L.

Published on: February 15, 2019

10.7K

Related Experiment Videos

Natural Product Discovery with LC-MS/MS Diagnostic Fragmentation Filtering: Application for Microcystin Analysis
07:18

Natural Product Discovery with LC-MS/MS Diagnostic Fragmentation Filtering: Application for Microcystin Analysis

Published on: May 31, 2019

9.0K
Isolation of Culturable Yeasts and Molds from Soils to Investigate Fungal Population Structure
10:33

Isolation of Culturable Yeasts and Molds from Soils to Investigate Fungal Population Structure

Published on: May 27, 2022

5.8K
Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the α-amylase Inhibitor from Lablab purpureus L.
09:21

Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the α-amylase Inhibitor from Lablab purpureus L.

Published on: February 15, 2019

10.7K

Area of Science:

  • Natural Product Chemistry
  • Medicinal Chemistry
  • Mycology

Background:

  • Aspergillus ustus is a fungal species known to produce a variety of bioactive secondary metabolites.
  • Austocystins are a class of fungal metabolites with diverse biological activities.
  • The exploration of novel compounds from microbial sources remains crucial for drug discovery.

Purpose of the Study:

  • To isolate and characterize new chlorinated austocystins from Aspergillus ustus NRRL 5856.
  • To elucidate the complex structures and absolute configurations of the isolated compounds.
  • To evaluate the immunosuppressive potential of the novel compounds.

Main Methods:

  • Isolation and purification of compounds using chromatographic techniques.
  • Structure elucidation via extensive spectroscopic analyses (NMR, MS).
  • Determination of absolute configurations using electronic circular dichroism (ECD) calculations and single-crystal X-ray diffraction.
  • Assessment of immunosuppressive activity using T cell proliferation (ConA-induced) and B cell proliferation (LPS-induced) assays.

    • Main Results:

    • Six new chlorinated austocystins, asperustins K-P (1-6), were isolated.
    • Asperustin K (1) possesses a unique octacyclic fused ring system, and asperustin L (2) features a novel hexacyclic skeleton.
    • Compounds 3-6 represent the first reported C-4'-O-C-5' bridged asperustin heterodimers.
    • Compounds 1, 3-6 demonstrated significant immunosuppressive activity with EC50 values in the low micromolar range.

    Conclusions:

    • The study successfully identified six novel chlorinated austocystins from Aspergillus ustus.
    • The unique structural features of asperustins K and L expand the chemical diversity of this metabolite class.
    • The identified heterodimers and other compounds exhibit promising immunosuppressive properties, warranting further investigation for therapeutic applications.