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Related Experiment Video

Updated: Sep 11, 2025

A High Throughput, Multiplexed and Targeted Proteomic CSF Assay to Quantify Neurodegenerative Biomarkers and Apolipoprotein E Isoforms Status

A High Throughput, Multiplexed and Targeted Proteomic CSF Assay to Quantify Neurodegenerative Biomarkers and Apolipoprotein E Isoforms Status

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Luminal Cerebrovascular Proteomics.

Sophia M Shi^1,2,3,4, Carolyn R Bertozzi^1,2,5, Tony Wyss-Coray^2,3,4,6

¹Department of Chemistry, Stanford University, Stanford, CA, USA.

|August 13, 2025

Summary

This summary is machine-generated.

Researchers developed a new in vivo proteomic method to map the brain

Keywords:

Aging Blood–brain barrier Brain endothelial cells CNS Luminal Neurodegeneration Proteomics Vasculature

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Area of Science:

Neuroscience
Proteomics
Cell Biology

Background:

Brain endothelial cells form the blood-brain barrier, crucial for central nervous system (CNS) homeostasis.
Distinct luminal and abluminal membrane compositions mediate cerebrovascular functions.
Current proteomic methods lack spatial resolution to differentiate these membrane compartments.

Purpose of the Study:

To develop a high-resolution in vivo proteomic strategy for profiling the luminal cerebrovascular surface.
To overcome limitations of existing techniques in discriminating between membrane compartments.

Main Methods:

In vivo perfusion of a membrane-impermeable biotinylation reagent to label luminal surface proteins.
Microvessel isolation and streptavidin-based enrichment of biotinylated proteins.
Downstream mass spectrometry (LC-MS/MS) for protein identification.

Main Results:

Successfully identified over 1,000 luminally localized proteins.
Achieved significantly improved enrichment of canonical luminal markers compared to conventional methods.
Generated a compartment-resolved atlas of the luminal cerebrovascular proteome.

Conclusions:

The developed method provides a high-confidence, spatially resolved map of the luminal cerebrovascular proteome.
Offers a scalable platform for studying endothelial surface biology in health and disease.
Enables investigation of aging-related changes and identification of therapeutic targets.