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Reprogramming White Fat Cells for Adipose Manipulation Transplantation (AMT) Therapy
Kelly An1,2,3, Yusuke Ito1,2,3, Nadav Ahituv1,2,3
1Department of Bioengineering and Therapeutic Sciences, University of California San Francisco, San Francisco, CA, USA.
Bio-Protocol
|August 13, 2025
Summary
This study introduces adipose manipulation transplantation (AMT), a method using CRISPR activation to genetically engineer adipocytes and adipose organoids. This technique enables therapeutic modifications for improved clinical outcomes, including cancer metabolic therapy.
Area of Science:
- Cell biology
- Genetics
- Endocrinology
Background:
- Adipocytes are key endocrine cells for energy storage and are utilized in clinical procedures like liposuction and transplantation.
- Ex vivo manipulation of adipocytes offers potential for therapeutic modifications and enhanced clinical outcomes post-transplantation.
Purpose of the Study:
- To provide a detailed protocol for modifying human adipocytes and adipose organoids using CRISPR activation (CRISPRa).
- To introduce adipose manipulation transplantation (AMT) as a novel technology for therapeutic genetic engineering of adipose tissue.
Main Methods:
- Utilized CRISPR activation (CRISPRa) for genetic engineering of human preadipocytes.
- Developed a protocol for generating modified adipocytes and adipose organoids.
Main Results:
- Successfully established a method for genetically engineering adipocytes and adipose organoids.
- Demonstrated the potential of these modified cells for therapeutic applications, such as cancer metabolic therapy.
Conclusions:
- Adipose manipulation transplantation (AMT) provides a viable platform for ex vivo genetic modification of adipocytes.
- This technology holds promise for developing novel therapeutic strategies, particularly in cancer treatment.
Keywords:
Adipocyte reprogrammingAdipocytesAdipose manipulation transplantation (AMT)Adipose organoidsCRISPR activationMetabolic cancer therapy
