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Related Experiment Video

Updated: Sep 11, 2025

Operation of a Benchtop Bioreactor
12:54

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BIO-SPEC: An open-source bench-top parallel bioreactor system.

Laurens Parret1, Kenneth Simoens1, Jo De Vrieze2,3

  • 1KU Leuven - Department of Chemical Engineering, Chemical and biochemical Reactor Engineering and Safety (CREaS), KU Leuven, Celestijnenlaan 200F box 2424, B-3001 Leuven, Belgium.

Hardwarex
|August 13, 2025
PubMed
Summary
This summary is machine-generated.

Researchers developed BIO-SPEC, an affordable, modular bioreactor system for microbial cultivation. This open-source hardware offers stable, long-term operation and flexible control, advancing accessible bioprocessing research.

Keywords:
BioreactorChemostatMicrobiologyOpen-sourceRaspberry PiSequencing batch

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Area of Science:

  • Biotechnology
  • Microbiology
  • Bioprocessing Engineering

Background:

  • High-cost commercial bioreactor systems limit accessibility in research and education.
  • Need for modular, cost-effective, and reliable bioreactor solutions for diverse cultivation strategies.

Purpose of the Study:

  • To design, construct, and validate BIO-SPEC, an open-source, bench-top bioreactor system.
  • To provide a flexible and affordable alternative to expensive commercial bioreactors for microbiology and bioprocessing.

Main Methods:

  • Development of a modular bioreactor system (BIO-SPEC) controlled by a Raspberry Pi.
  • Integration of thermoelectric condensers for stable, chiller-free operation.
  • Design validation through batch, sequencing batch, and chemostat cultivation experiments.

Main Results:

  • Successful construction and validation of the BIO-SPEC system.
  • Demonstrated stable long-term operation without external chillers.
  • Verified flexibility in headplate design, gas supply, and feeding strategies.

Conclusions:

  • BIO-SPEC offers a cost-effective, versatile, and reliable platform for microbial cultivation.
  • The open-source nature of BIO-SPEC promotes accessibility and innovation in bioprocessing research.
  • This system empowers researchers with advanced capabilities at a fraction of commercial system costs.