Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

5.3K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
5.3K
Ribosome Profiling02:24

Ribosome Profiling

3.6K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.6K
Extraction: Advanced Methods00:56

Extraction: Advanced Methods

529
Metal ions can be separated from one another by complexation with organic ligands–the chelating agent– to form uncharged chelates. Here, the chelating agent must contain hydrophobic groups and behave as a weak acid, losing a proton to bind with the metal. Since most organic ligands used in this process are insoluble or undergo oxidation in the aqueous phase, the chelating agent is initially added to the organic phase and extracted into the aqueous phase. The metal-ligand complex is...
529
Imaging Studies II: Positron Emission Tomography and Scintigraphy01:25

Imaging Studies II: Positron Emission Tomography and Scintigraphy

235
Positron Emission Tomography (PET) is a medical imaging technique that provides crucial insights into the body's physiological functions at a molecular level. It is an indispensable resource for diagnosing, staging, and monitoring various illnesses, notably cancer, neurological disorders, and cardiovascular conditions.
Fundamental Principles of PET
235

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Epithelial-mesenchymal transition and immunosuppression: Two sides of the same coin.

Cellular and molecular life sciences : CMLS·2026
Same author

Identifying and targeting abnormal mitochondrial localization associated with psychosis.

bioRxiv : the preprint server for biology·2026
Same author

Scalable data harmonization for single-cell image-based profiling with CytoTable.

Patterns (New York, N.Y.)·2026
Same author

CD4+ T Cells Sensitize Quasimesenchymal Breast Tumors Lacking CD73 to Anti-CTLA4 Immune Checkpoint Blockade Therapy.

Cancer research communications·2026
Same author

Single-cell hit calling in high-content imaging screens with Buscar.

bioRxiv : the preprint server for biology·2026
Same author

Progress and new challenges in image-based profiling.

Molecular systems biology·2026
Same journal

Poisoning the Genome: Targeted Backdoor Attacks on DNA Foundation Models.

ArXiv·2026
Same journal

Mechanistic mathematical model of the in vitro infection dynamics of Bunyamwera and Batai viruses including MOI-dependent shortening of the eclipse phase.

ArXiv·2026
Same journal

AI-Driven Lumped-Element Modeling of Human Respiratory System for Studying Voice Mechanics.

ArXiv·2026
Same journal

Beyond Algorithms: Conceptual Innovation in Medical Imaging AI.

ArXiv·2026
Same journal

Feynman Kac Reweighted Schrödinger Bridge Matching for Surface-Based Tau PET Harmonization.

ArXiv·2026
Same journal

Agentic Discovery of Non-Canonical Antimicrobial Peptides with AMPGAN v3.

ArXiv·2026
See all related articles

Related Experiment Video

Updated: Sep 11, 2025

Introduction of an Integrated Pathology Image Management, Artificial Intelligence, and Reporting System
05:33

Introduction of an Integrated Pathology Image Management, Artificial Intelligence, and Reporting System

Published on: July 11, 2025

244

Progress and new challenges in image-based profiling.

Erik Serrano1, John Peters2, Jesko Wagner3

  • 1Department of Biomedical Informatics, University of Colorado School of Medicine.

Arxiv
|August 13, 2025
PubMed
Summary
This summary is machine-generated.

Image-based profiling uses microscopy data for cellular analysis, revolutionizing drug discovery. Advances in deep learning and open-source tools enhance its power, but challenges remain for future development.

More Related Videos

A Multimodal Imaging Framework to Advance Phenotyping of Living Label-free Breast Cancer Cells
10:37

A Multimodal Imaging Framework to Advance Phenotyping of Living Label-free Breast Cancer Cells

Published on: August 22, 2025

171
Author Spotlight: Generating Neuronal Phenotypic Profiles - A Protocol to Culture and Image Human Midbrain Dopaminergic Neurons
09:21

Author Spotlight: Generating Neuronal Phenotypic Profiles - A Protocol to Culture and Image Human Midbrain Dopaminergic Neurons

Published on: July 7, 2023

1.6K

Related Experiment Videos

Last Updated: Sep 11, 2025

Introduction of an Integrated Pathology Image Management, Artificial Intelligence, and Reporting System
05:33

Introduction of an Integrated Pathology Image Management, Artificial Intelligence, and Reporting System

Published on: July 11, 2025

244
A Multimodal Imaging Framework to Advance Phenotyping of Living Label-free Breast Cancer Cells
10:37

A Multimodal Imaging Framework to Advance Phenotyping of Living Label-free Breast Cancer Cells

Published on: August 22, 2025

171
Author Spotlight: Generating Neuronal Phenotypic Profiles - A Protocol to Culture and Image Human Midbrain Dopaminergic Neurons
09:21

Author Spotlight: Generating Neuronal Phenotypic Profiles - A Protocol to Culture and Image Human Midbrain Dopaminergic Neurons

Published on: July 7, 2023

1.6K

Area of Science:

  • Computational biology
  • Cellular imaging analysis

Background:

  • Image-based profiling has been pivotal in cellular phenotype analysis for over 20 years.
  • It transforms high-throughput microscopy data into unbiased measurements for various biological applications.

Purpose of the Study:

  • To review the computational evolution of image-based profiling.
  • To detail current methods, limitations, and future directions in the field.

Main Methods:

  • Review of computational advancements, including deep learning for feature extraction and data integration.
  • Incorporation of single-cell analysis and batch effect correction techniques.
  • Analysis of open-source software ecosystems and community standards.

Main Results:

  • Deep learning has significantly improved feature extraction, scalability, and multimodal data integration.
  • Single-cell transcriptomics-inspired methods enhance analytical precision.
  • Open-source platforms and community standards increase accessibility and collaboration.

Conclusions:

  • Image-based profiling is rapidly advancing, driven by computational innovations.
  • Despite progress, significant challenges require further research and development.
  • This review provides a roadmap for researchers navigating the field's technical landscape.