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Related Experiment Video

Updated: Sep 11, 2025

A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli

A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli

Published on: December 9, 2017

Targeted Protein Acetylation Through Chemically Induced Proximity.

Wesley W Wang¹, Soumya Jyoti Singha Roy¹, Christopher G Parker¹

¹Department of Chemistry, The Scripps Research Institute, La Jolla, California 92037, United States.

Accounts of Chemical Research

|August 13, 2025

Summary

Researchers developed AceTAG, a chemical biology tool for precise protein acetylation in cells. This method enhances protein stability and function, offering new avenues for disease research and potential therapies.

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Last Updated: Sep 11, 2025

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Area of Science:

Biochemistry and Chemical Biology
Molecular and Cellular Biology
Post-Translational Modifications

Background:

Protein acetylation is a crucial post-translational modification regulating cellular processes.
Dysregulation of acetylation is linked to diseases like cancer and metabolic disorders.
Current tools lack precision for studying site-specific acetylation in live cells.

Purpose of the Study:

To introduce AceTAG, a chemically induced proximity platform for targeted protein acetylation.
To demonstrate the utility of AceTAG for precise, tunable, and dynamic acetylation in live cells.
To explore the potential of AceTAG for functional investigations and therapeutic applications.

Main Methods:

Development of AceTAG, a heterobifunctional ligand system.
Recruitment of endogenous lysine acetyltransferases (KATs) to specific protein targets.
Application of AceTAG to proteins including histone H3.3, p65/RelA, and p53.

Main Results:

AceTAG enables selective, tunable, and dynamic protein acetylation.
Chemically induced acetylation of p53 enhanced its stability and transcriptional activity.
AceTAG proved effective across diverse protein targets, including p53 mutants.

Conclusions:

AceTAG is a powerful chemical tool for investigating protein acetylation biology.
Targeted acetylation using AceTAG holds therapeutic potential for acetylation-related diseases.
This platform advances the study of post-translational modifications in live cellular systems.