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Andrographis paniculata Extract Supports Skin Homeostasis by Enhancing Epidermal Stem Cell Function and Reinforcing
Roberta Lotti1, Laetitia Cattuzzato2, Xuefeng Huang3
1DermoLAB, Surgical, Medical, Dental and Morphological Sciences Department, University of Modena and Reggio Emilia, 41124 Modena, Italy.
Cells
|August 13, 2025
Summary
Andrographis paniculata extract (APE) improves skin aging by enhancing the dermo-epidermal junction and preserving keratinocyte stem cell potential. This natural compound supports skin regeneration and repair for anti-aging benefits.
Area of Science:
- Dermatology
- Cell Biology
- Natural Product Chemistry
Background:
- Skin aging involves decreased epidermal homeostasis and dermo-epidermal junction (DEJ) integrity.
- Reduced stem cell potential and impaired tissue regeneration are key features of aged skin.
Purpose of the Study:
- To investigate the effects of Andrographis paniculata extract (APE) on keratinocyte stem cells (KSCs) and DEJ composition in human skin.
- To evaluate APE's potential as a skin anti-aging strategy targeting stem cell maintenance and extracellular matrix stability.
Main Methods:
- Utilized human skin explants and cell culture models to assess APE's impact.
- Analyzed DEJ composition markers (Collagen IV, Laminin, MMP-9) and KSC stemness markers (collagen XVII, β1-integrin, Ki-67, KRT15, Survivin, LRIG1).
- Employed a co-culture model simulating UVB-induced DEJ damage.
Main Results:
- APE enhanced DEJ composition by increasing Collagen IV and Laminin, and decreasing MMP-9.
- APE preserved KSC stemness potential by upregulating relevant markers.
- APE selectively stimulated KSC proliferation and promoted Collagen IV and Laminin secretion.
- APE increased Laminin production in KSCs under simulated photo-damage conditions.
Conclusions:
- Andrographis paniculata extract (APE) demonstrates significant potential for skin anti-aging applications.
- APE improves DEJ integrity and maintains KSC stemness, promoting skin regeneration and repair.
- APE offers a promising natural approach to combat skin aging by targeting key cellular and matrix components.
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