Fucoidan Modulates Osteoarthritis Progression Through miR-22/HO-1 Pathway
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View abstract on PubMed
Summary
This summary is machine-generated.Fucoidan protects against osteoarthritis (OA) by reducing oxidative stress and inflammation. It modulates the miR-22/heme oxygenase-1 pathway, offering a potential therapeutic strategy for OA treatment.
Area Of Science
- Biochemistry
- Cell Biology
- Pharmacology
Background
- Osteoarthritis (OA) is a degenerative joint disease causing significant disability in the elderly.
- Interleukin-1 beta (IL-1β) plays a key role in OA pathogenesis by inducing articular tissue damage.
- Fucoidan, a sulfated polysaccharide, possesses anti-inflammatory and antioxidant properties with potential therapeutic applications in OA.
Purpose Of The Study
- To investigate the protective effects of fucoidan against IL-1β-induced damage in human chondrocytes and synovial fibroblasts.
- To elucidate the underlying molecular mechanisms, focusing on the role of microRNAs (miRs) and heme oxygenase-1 (HO-1).
- To validate the findings in an in vivo rat model of OA.
Main Methods
- Human chondrocytes and synovial fibroblasts were treated with fucoidan prior to IL-1β stimulation.
- Oxidative stress markers and catabolic enzyme levels were measured to assess fucoidan's protective effects.
- An OA rat model was used for in vivo validation.
- In silico and experimental methods, including luciferase reporter assays, were employed to identify and validate miR-HO-1 interactions.
Main Results
- Fucoidan demonstrated protective effects against IL-1β-induced oxidative stress and catabolic processes in vitro and in vivo.
- Fucoidan treatment normalized HO-1 expression and reduced inducible nitric oxide synthase and matrix metalloproteinase levels.
- A novel mechanism involving fucoidan's modulation of the miR-22/HO-1 pathway in OA was identified, with miR-22 upregulation by IL-1β and subsequent attenuation by fucoidan.
Conclusions
- Fucoidan effectively mitigates OA-related oxidative stress in joint cells via the miR-22/HO-1 axis.
- The miR-22/HO-1 pathway is a critical therapeutic target for osteoarthritis.
- Fucoidan represents a promising therapeutic agent for managing osteoarthritis.
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