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Related Concept Videos

Notch Signaling Pathway03:14

Notch Signaling Pathway

4.4K
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
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Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

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Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
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Hedgehog Signaling Pathway02:33

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The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
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Determination01:51

Determination

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During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In...
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Related Experiment Video

Updated: Sep 11, 2025

Micro-dissection of Enamel Organ from Mandibular Incisor of Rats Exposed to Environmental Toxicants
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Notch2 Deletion Compromises Epithelial Integrity and Enamel Formation in Rodent Incisors.

Argyro Lamprou1, Cristina Porcheri1, Thimios A Mitsiadis1,2

  • 1Institute of Oral Biology, Centre of Dental Medicine, Faculty of Medicine, University of Zurich, 8032 Zurich, Switzerland.

Cells
|August 13, 2025
PubMed
Summary

Notch2 signaling is crucial for rodent incisor development. Its deletion disrupts dental epithelium organization, enamel structure, and tooth eruption, highlighting Notch2

Keywords:
Notch signallingNotch2cell fatesdental epitheliumenamelintercellular junctionsrodent incisorstoothtransgenic mice

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Area of Science:

  • Developmental Biology
  • Cell Signaling
  • Oral Biology

Background:

  • The Notch signaling pathway is essential for cell fate determination and organ development.
  • Rodent incisors continuously erupt, requiring precise regulation of epithelial stem cells.

Purpose of the Study:

  • To investigate the specific role of the Notch2 receptor in the development and function of rodent incisor epithelial cells.
  • To elucidate the impact of Notch2 signaling on dental epithelium organization and enamel formation.

Main Methods:

  • Utilized transgenic mouse models (Notch1-CreERT2 and Notch2-CreERT2) to trace cell lineages.
  • Examined Notch2-deficient mice (K14-Cre; Notch2fl/fl) using immunofluorescence, gene expression, micro-CT, and scanning electron microscopy.
  • Validated findings through in vitro Notch signaling inhibition studies.

Main Results:

  • Notch2 deletion led to smaller incisors with disorganized dental epithelium and defective enamel.
  • Delayed incisor eruption correlated with altered proliferation and differentiation of cervical loop epithelial stem cells.
  • In vitro inhibition of Notch signaling mimicked the in vivo phenotype.

Conclusions:

  • Notch2 plays a critical role in epithelial cell fate acquisition during incisor development.
  • Notch2 signaling is essential for maintaining dental epithelium organization and enamel structure.
  • This study establishes Notch2 as a key regulator of rodent incisor morphogenesis and function.