Deep learning-based frameworks for the detection and classification of soniferous fish

Passive acoustic monitoring (PAM) is emerging as a valuable tool for assessing fish populations in natural habitats. This study compares two deep learning-based frameworks: (1) a multi-label segmentation-based classification system (SegClas) combining convolutional neural networks and long short term memory networks and, (2) an object detection approach (ObjDet) using a You Only Look Once based model to detect, classify, and count sounds produced by soniferous fish in the Tagus Estuary, Portugal. The target species-Lusitanian toadfish (Halobatrachus didactylus), meagre (Argyrosomus regius), and weakfish (Cynoscion regalis)-exhibit overlapping vocalization patterns, posing classification challenges. Results show both methods achieve high accuracy (over 96%) and F1 scores above 87% for species-level sound identification, demonstrating their effectiveness under varied noise conditions. ObjDet generally offers slightly higher classification performance (F1 up to 92%) and can annotate each vocalization for more precise counting. However, it requires bounding-box annotations and higher computational costs (inference time of ca. 1.95 s/h of recording). In contrast, SegClas relies on segment-level labels and provides faster inference (ca. 1.46 s/h). This study also compares both counting strategies, each offering distinct advantages for different ecological and operational needs. Our results highlight the potential of deep learning-based PAM for fish population assessment.