Association between miR499b A>G gene polymorphism and recurrent pregnancy loss in Turkish women

Recurrent pregnancy loss is characterized as a disorder characterized by two or more miscarriages, and its etiology is unclear. Recent research has focused on microRNA and recurrent pregnancy loss; however, single-nucleotide polymorphisms of microRNA in recurrent pregnancy loss need to be better understood. The aim of the study was to assess if there is any relationship between the miR499b A>G gene polymorphism and recurrent pregnancy loss in Turkish women.

The work has 267 participants, including 153 patients in the recurrent pregnancy loss and 114 participants in the control group. DNA isolation from peripheral blood was conducted using a kit-based approach, followed by the implementation of polymerase chain reaction and restriction fragment length polymorphism methods as outlined in the procedure.

The rates of recurrent pregnancy loss were determined as 32.7% for the AA genotype, 51.6% for the AG genotype, and 15.7% for the GG genotype. When the AA genotype type was taken as a reference, the risk of recurrent pregnancy loss was 0.922 times higher (95%CI 0.513-1.655; p=0.785) in the AG type and 0.354 times lower (95%CI 0.178-0.705; p=0.003) in the GG type. In the recurrent pregnancy loss patient group, the GG genotype was lower than the expected value. The GG genotype was found to be less susceptible to recurrent pregnancy loss development.

Based on our results, GG genotype frequencies, which are the recessive model of the miR499b A>G gene polymorphism, may be a protective genotype in susceptibility to recurrent pregnancy loss in Turkish women. The results obtained from this study represent the first data to be established for the Turkish population.