Related Experiment Video
Updated: Sep 11, 2025

Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production
Published on: March 2, 2014
Insect dome receptor directly recognizes viral envelopes to activate JAK/STAT signaling and induce conserved
Siyu Chen1, Yupeng Tang1, Chunyu Zhang1
1State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Abstract:
Insect Janus kinase/signal transducer and activator of transcription (JAK/STAT) pathway plays a crucial role in antiviral defense, yet how arboviruses activate this pathway remains unclear. Here, we reveal that outer capsid protein of a double-stranded RNA (dsRNA) arbovirus (rice gall dwarf virus, RGDV) directly binds the Dome receptor's extracellular domain, triggering receptor dimerization and JAK/STAT activation. Structural and mutational analyses pinpoint cysteine 743 in Dome as essential for viral recognition, establishing Dome as the first identified viral pattern recognition receptor (PRR) in insects capable of initiating JAK/STAT signaling, a pathway previously thought to be exclusively cytokine dependent. Furthermore, we identify viperin as a transcriptional factor STAT-regulated antiviral effector with broad-spectrum activity against multiple rice viruses. Intriguingly, RGDV's nonstructural protein Pns11 competitively binds STAT, blocking its phosphorylation by JAK and nuclear translocation, thereby suppressing viperin expression. These findings elucidate how leafhopper Dome functions as a viral PRR and how arboviruses antagonize Dome-JAK-STAT signaling to promote persistent viral infection.
More Related Videos
06:44Confocal Imaging of Double-Stranded RNA and Pattern Recognition Receptors in Negative-Sense RNA Virus Infection
Published on: January 26, 2019
12:43Monitoring Activation of the Antiviral Pattern Recognition Receptors RIG-I And PKR By Limited Protease Digestion and Native PAGE
Published on: July 29, 2014
Related Concept Videos
Immune Response Against Viral Pathogens
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
The JAK-STAT Signaling Pathway
Receptor Downregulation in MVBs
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
Viral Structure
Antigen Processing Pathways
MHC Class I: Presenting Endogenous...
Regulation of the Unfolded Protein Response