Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Base pairing involving deoxyinosine: implications for probe design.

F H Martin, M M Castro, F Aboul-ela

    Nucleic Acids Research
    |December 20, 1985
    PubMed
    Summary

    Deoxyinosine (I) base pairs show varying thermal stability in DNA duplexes, with I:C pairs being less stable than A:T pairs. Neighboring bases significantly impact stability, influencing DNA probe design.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Quantification of miltefosine in peripheral blood mononuclear cells by high-performance liquid chromatography-tandem mass spectrometry.

    Journal of chromatography. B, Analytical technologies in the biomedical and life sciences·2015
    Same author

    Addressing key ecological questions to support policy-making in Brazil.

    Brazilian journal of biology = Revista brasleira de biologia·2013
    Same author

    Inhibitory effects of caspase inhibitors on the activity of matrix metalloproteinase-2.

    Biochemical pharmacology·2013
    Same author

    Prevalence of human papillomavirus (HPV) type 16 variants and rare HPV types in the central Amazon region.

    Genetics and molecular research : GMR·2011
    Same author

    Spironolactone and hydrochlorothiazide exert antioxidant effects and reduce vascular matrix metalloproteinase-2 activity and expression in a model of renovascular hypertension.

    British journal of pharmacology·2010
    Same author

    The Evolution of Clinical Medicine and Surgery in Relation to the Preservation of Health and Life.

    Canadian Medical Association journal·2010

    Area of Science:

    • Molecular Biology
    • Biochemistry
    • Oligonucleotide Chemistry

    Background:

    • Deoxyinosine (I) is an important modified nucleoside in DNA.
    • Understanding the base-pairing stability of deoxyinosine is crucial for various applications, including DNA probe design.

    Purpose of the Study:

    • To determine the thermal stability of oligodeoxyribonucleotide duplexes containing deoxyinosine (I) paired with A, T, C, or G.
    • To investigate the influence of neighboring bases on the stability of I-containing base pairs.

    Main Methods:

    • Optical melting techniques were employed to measure the thermal stability of DNA duplexes.
    • Oligonucleotides with specific sequences containing deoxyinosine were synthesized and analyzed.

    Main Results:

    • The relative stabilities of I-containing base pairs were determined, revealing I:C pairs are less stable than A:T pairs.
    • Significant neighboring-base effects were observed, with stability varying greatly depending on adjacent bases.
    • The order of stability, independent of sequence effects, was found to be I:C > I:A > I:T ≈ I:G.

    Conclusions:

    • Deoxyinosine base pairs exhibit distinct stability profiles compared to standard DNA base pairs.
    • Neighboring base context plays a critical role in modulating the stability of deoxyinosine-containing DNA duplexes.
    • These findings have implications for the rational design of DNA oligonucleotide probes and other nucleic acid-based technologies.

    Related Experiment Videos