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Related Experiment Videos

Interaction of DAPI with double-stranded ribonucleic acids.

G Manzini, L Xodo, M L Barcellona

    Nucleic Acids Research
    |December 20, 1985
    PubMed
    Summary
    This summary is machine-generated.

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    Biophysical chemistry·2012

    DAPI binds to both RNA and DNA, showing high affinity for AU-rich regions and enhancing fluorescence. Caution is advised when using DAPI as a selective DNA stain in the presence of RNA.

    Area of Science:

    • Biophysical Chemistry
    • Molecular Biology
    • Fluorescent Probes

    Background:

    • 4',6-diamidino-2-phenylindole (DAPI) is a fluorescent stain commonly used for DNA visualization.
    • Previous studies indicated DAPI's interaction with double-stranded polydeoxynucleotides.

    Purpose of the Study:

    • To investigate the interaction of DAPI with natural and synthetic double-stranded polyribonucleotides.
    • To compare DAPI's binding modes with RNA versus DNA.

    Main Methods:

    • Optical spectroscopy
    • Calorimetric methods
    • Fluorescence enhancement measurements

    Main Results:

    • DAPI exhibits two interaction modes with polyribonucleotides, similar to polydeoxynucleotides.

    Related Experiment Videos

  • High-affinity binding to AU-rich sequences leads to significant fluorescence enhancement.
  • A narrow groove binding model involving hydrogen bonds with AU pairs is proposed.

    • Conclusions:

    • DAPI interacts with both RNA and DNA, not exclusively DNA.
    • The findings suggest caution when using DAPI for selective DNA staining in the presence of RNA.
    • Intercalation is unlikely due to DAPI's non-planar structure.