Recent Advances in Engineering the Unfolded Protein Response in Recombinant Chinese Hamster Ovary Cell Lines
These videos have been matched automatically. Contact us if they are not relevant.
These videos have been matched automatically. Contact us if they are not relevant.
View abstract on PubMed
Summary
This summary is machine-generated.Chinese hamster ovary (CHO) cells manage endoplasmic reticulum (ER) stress via the unfolded protein response (UPR) for biopharmaceutical production. Optimizing UPR and ER stress is key to enhancing protein titers and quality in CHO cell fermentation.
Area Of Science
- Biotechnology
- Cell Biology
- Bioprocess Engineering
Background
- Chinese hamster ovary (CHO) cells are crucial for glycosylated biopharmaceutical production.
- High production demands can lead to endoplasmic reticulum (ER) stress and the unfolded protein response (UPR).
- ER stress is a significant bottleneck in secreting therapeutic proteins.
Purpose Of The Study
- To review ER stress markers and the optimal UPR profile in high-producing CHO cells.
- To discuss the context-dependency of UPR profiles in recombinant CHO cell lines.
- To elucidate recent strategies for engineering ER stress responses in CHO cells for bioprocessing.
Main Methods
- Review of common ER stress-related markers.
- Discussion of optimal UPR profiles for high-producing CHO cells.
- Analysis of bioprocess operations and strategies for controlling ER stress.
Main Results
- The unfolded protein response (UPR) is a cellular defense mechanism against ER stress.
- Prolonged UPR can lead to apoptosis, impacting protein production.
- Engineering ER stress management is vital for optimizing CHO cell fermentation.
Conclusions
- Optimizing UPR and ER stress management in CHO cells is critical for high-quality biopharmaceutical production.
- Bioprocess engineering and targeted strategies can improve cellular responses to ER stress.
- Future research should focus on further engineering the CHO cell UPR for enhanced biomanufacturing.
These videos have been matched automatically. Contact us if they are not relevant.
These videos have been matched automatically. Contact us if they are not relevant.