Efficient Assessment and Optimisation of Medium Components Influencing Extracellular Xylanase Production by...

Area of Science:

• Biotechnology
• Enzyme Engineering
• Microbial Production

Background:

• Xylanase is crucial for various industries but faces high production costs and safety concerns.
• Renewable polymers offer a cost-effective and safer alternative for enzyme production.
• Lactic acid bacteria (LAB), like Pediococcus pentosaceus G4, are promising non-pathogenic sources for enzyme production.

Purpose of the Study:

• To optimize extracellular xylanase production by Pediococcus pentosaceus G4.
• To identify key medium components influencing xylanase yield using statistical methods.
• To develop a cost-effective production strategy for industrial xylanase applications.

Main Methods:

• Utilized Plackett-Burman Design (PBD) to screen significant medium components.
• Employed Central Composite Design (CCD) to optimize concentrations of key factors.
• Assessed extracellular xylanase activity and production costs.

Main Results:

• PBD identified glucose, almond shell, peanut shell, urea, and magnesium sulfate as significant factors.
• CCD optimization yielded a 3.13-fold increase in specific xylanase activity.
• Production costs decreased by 7.99-fold compared to commercial medium, achieving 2.765 U/mg activity.

Conclusions:

• Statistical optimization effectively enhances extracellular xylanase production in P. pentosaceus G4.
• Optimized renewable polymer-based medium offers a cost-effective solution for industrial enzyme production.
• LAB present a viable and safe alternative for producing hemicellulolytic enzymes.