Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Pulmonary Tuberculosis IV01:26

Pulmonary Tuberculosis IV

Tuberculosis, more commonly referred to as TB, is an infectious disease stemming from Mycobacterium tuberculosis. While it primarily impacts the lungs, TB can also affect other body areas. Given its severity and global impact, timely and accurate diagnosis is crucial for controlling its spread and improving patient outcomes.
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...
Drug Toxicity: Allergic Reactions01:30

Drug Toxicity: Allergic Reactions

Drug-related allergies are immune-mediated responses triggered by the administration of pharmacological agents. These hypersensitivity reactions are classified based on the immune mechanisms involved. The four primary types—Type I, II, III, and IV—are mediated by different immunological pathways and exhibit distinct clinical manifestations.Type I Hypersensitivity/ IgE-Mediated Reactions: Immunoglobulin E (IgE) immediately mediates Type I hypersensitivity reactions. Upon initial exposure to a...
Rocky Mountain Spotted Fever01:26

Rocky Mountain Spotted Fever

Rocky Mountain Spotted Fever (RMSF) is a severe tick-borne illness caused by Rickettsia rickettsii, a Gram-negative, coccobacillary bacterium. This pathogen is an obligate intracellular parasite, requiring a host cell for replication. Transmission occurs through the bite of an infected tick. In the United States, the most important vectors are Dermacentor variabilis (American dog tick) and Dermacentor andersoni (Rocky Mountain wood tick), though other tick species may also serve as vectors.
Diphtheria01:28

Diphtheria

Diphtheria is an acute, toxin-mediated infectious disease that primarily affects the upper respiratory tract. It is caused by Corynebacterium diphtheriae, a Gram-positive, pleomorphic rod that lacks spore-forming capability and exhibits a characteristic club-shaped morphology under microscopic examination. While C. diphtheriae can asymptomatically colonize mucosal surfaces, clinical disease manifests only when the bacterial strain is lysogenized by a specific β-corynephage. This phage...
Rabies01:28

Rabies

Rabies is a lethal zoonotic disease caused by a single-stranded, negative-sense RNA virus of the Lyssavirus genus, within the family Rhabdoviridae. Its primary mode of transmission to humans is through bites or saliva-contaminated scratches from infected mammals such as dogs, bats, raccoons, or foxes. Transmission can also occur if infectious saliva contacts abraded skin or intact mucous membranes, including the conjunctiva.Viral Entry and Early ReplicationOnce introduced at the bite or scratch...
Hypersensitivity Reactions: Immune-Complex Reactions01:19

Hypersensitivity Reactions: Immune-Complex Reactions

Type III hypersensitivity reactions occur when antigen–antibody complexes form and activate the complement system. Normally, these complexes help the clearance of antigens by phagocytes and red blood cells. However, when large numbers of immune complexes are present, they can deposit in tissues—particularly in the walls of blood vessels—leading to inflammation and tissue injury. These deposits trigger complement activation and neutrophil recruitment, resulting in serum sickness, a systemic...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rapidly progressive dementia with parkinsonism in the Italian CJD Registry: underlying pathology and clinical-biomarker clues for differential diagnosis.

Parkinsonism & related disorders·2026
Same author

"Polyradiculoneuritis" as an Atypical Clinical Presentation of Creutzfeldt-Jakob Disease: A Case Report and Review of Literature.

Life (Basel, Switzerland)·2026
Same author

Tear fluid as a novel specimen for detection of alpha-synuclein seeding activity in Parkinson's disease.

Scientific reports·2026
Same author

The Phenotypic Spectrum of Sporadic Creutzfeldt-Jakob Disease Cortical Subtype.

Annals of neurology·2025
Same author

Reviewer Comment on Slota et al. "Diagnosis of Creutzfeldt-Jakob Disease in Canada: An Update on Cerebrospinal Fluid Testing from 2016-2024".

The Canadian journal of neurological sciences. Le journal canadien des sciences neurologiques·2025
Same author

Clinical phenotype associated with A118V mutation of PRPN gene.

Journal of neurology·2025

Related Experiment Video

Updated: Jun 19, 2026

Monitoring Immune Cells Trafficking Fluorescent Prion Rods Hours after Intraperitoneal Infection
09:22

Monitoring Immune Cells Trafficking Fluorescent Prion Rods Hours after Intraperitoneal Infection

Published on: November 19, 2010

11.7K

Sciatic Integrity Is Necessary for Fast and Efficient Scrapie Infection After Footpad Injection.

Franco Cardone1, Flavia Porreca1, Marco Sbriccoli1

  • 1Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy.

International Journal of Molecular Sciences
|August 14, 2025
PubMed
Summary
This summary is machine-generated.

Prion diseases spread rapidly to the central nervous system (CNS) via peripheral nerves. This study reveals intranerval prion transit is faster than previously thought, offering new therapeutic targets.

Keywords:
263Kintraneural transitneuroinvasionneurotropic pathogensprionscrapie

More Related Videos

An In Vivo Murine Sciatic Nerve Model of Perineural Invasion
09:55

An In Vivo Murine Sciatic Nerve Model of Perineural Invasion

Published on: April 23, 2018

12.3K
Mouse Footpad Inoculation Model to Study Viral-Induced Neuroinflammatory Responses
09:07

Mouse Footpad Inoculation Model to Study Viral-Induced Neuroinflammatory Responses

Published on: June 14, 2020

11.0K

Related Experiment Videos

Last Updated: Jun 19, 2026

Monitoring Immune Cells Trafficking Fluorescent Prion Rods Hours after Intraperitoneal Infection
09:22

Monitoring Immune Cells Trafficking Fluorescent Prion Rods Hours after Intraperitoneal Infection

Published on: November 19, 2010

11.7K
An In Vivo Murine Sciatic Nerve Model of Perineural Invasion
09:55

An In Vivo Murine Sciatic Nerve Model of Perineural Invasion

Published on: April 23, 2018

12.3K
Mouse Footpad Inoculation Model to Study Viral-Induced Neuroinflammatory Responses
09:07

Mouse Footpad Inoculation Model to Study Viral-Induced Neuroinflammatory Responses

Published on: June 14, 2020

11.0K

Area of Science:

  • Neuroscience
  • Infectious Diseases
  • Prion Biology

Background:

  • Prion diseases infect hosts via peripheral routes and spread to the central nervous system (CNS) through peripheral nerves.
  • Understanding prion neuroinvasion mechanisms is crucial for developing therapeutic strategies.

Purpose of the Study:

  • To investigate the intranerval transit mechanism of 263K scrapie prions in golden Syrian hamsters (GSHs).
  • To determine the speed and entry points of prion spread within peripheral nerves.

Main Methods:

  • Transection of the sciatic nerve at varying times post-footpad injection in GSHs.
  • Recording incubation periods to estimate prion infection efficiency.
  • In vivo exposure of sciatic nerves to 263K infectivity.

Main Results:

  • Intranerval transit of 263K scrapie prions is significantly faster than previously reported, reaching speeds up to 50 mm/day.
  • Prion entry into nerves occurs primarily through nerve terminals, not direct sciatic nerve contact.
  • The experimental approach provides a method to verify neuroinvasion timing.

Conclusions:

  • Prion neuroinvasion via peripheral nerves is a rapid process.
  • Prion entry at nerve terminals suggests potential targets for early therapeutic intervention.
  • This methodology can be applied to study neuroinvasion in various prion diseases.