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Screening Method for the Selection of Oleaginous Yeast-Producing Gold Nanoparticles
Jesus D Guerra1, Diana Mariscal-Nava1, Miguel Avalos-Borja2
1LIBBA Laboratory, Industrial Biotechnology Unit, Research and Assistance Center in Technology and Design of the State of Jalisco, A.C., Av. Normalistas No. 800 Col. Colimas de la Normal, C.P., Guadalajara 44270, Jalisco, Mexico.
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The demand for eco-friendly nanomaterial synthesis has increased interest in biological approaches. Yeast-mediated biosynthesis of gold nanoparticles (AuNPs) offers a sustainable alternative with potential biotechnological applications. This study developed a rapid screening method to identify oleaginous yeast strains able to synthesize AuNPs. A collection of 114 oleaginous yeasts from the LIBBA laboratory was screened. UV-Vis spectroscopy at 530-560 nm was used to assess nanoparticle formation, identifying 20 strains that effectively synthesize AuNP. Electron microscopy confirmed the presence of intracellular and extracellular nanoparticles, with variations in size and morphology. This screening and optimization approach effectively identified promising yeast candidates and refined biosynthesis conditions, providing a foundation for industrial-scale nanoparticle production.
