Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

1.9K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
1.9K
Gene Therapy00:59

Gene Therapy

25.9K
Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be...
25.9K
Microorganisms in Medicine and Therapeutics01:29

Microorganisms in Medicine and Therapeutics

330
Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.
330
Transgenic Organisms00:53

Transgenic Organisms

31.6K
Overview
31.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Advances in ex vivo expansion of hematopoietic stem and progenitor cells for clinical applications.

Frontiers in bioengineering and biotechnology·2024
Same author

Dual production of human mesenchymal stromal cells and derived extracellular vesicles in a dissolvable microcarrier-based stirred culture system.

Cytotherapy·2024
Same author

A passage-free, simplified, and scalable novel method for iPSC generation in three-dimensional culture.

Regenerative therapy·2024
Same author

Natural killer cells suppress human cutaneous squamous cell carcinoma cancer cell survival and tumor growth.

Molecular carcinogenesis·2023
Same author

A Dynamic 3D Aggregate-Based System for the Successful Expansion and Neural Induction of Human Pluripotent Stem Cells.

Frontiers in cellular neuroscience·2022
Same author

Current Perspectives on "Off-The-Shelf" Allogeneic NK and CAR-NK Cell Therapies.

Frontiers in immunology·2021

Related Experiment Video

Updated: Sep 11, 2025

Small RNA Transfection in Primary Human Th17 Cells by Next Generation Electroporation
10:15

Small RNA Transfection in Primary Human Th17 Cells by Next Generation Electroporation

Published on: April 13, 2017

8.0K

Transfection Technologies for Next-Generation Therapies.

Dinesh Simkhada1, Su Hui Catherine Teo1, Nandu Deorkar1

  • 1Biopharma and Bioproduction, Cell and Gene Therapy, Avantor, 77 Corporate Drive, Bridgewater, NJ 08807, USA.

Journal of Clinical Medicine
|August 14, 2025
PubMed
Summary
This summary is machine-generated.

Emerging non-viral transfection technologies, including lipid nanoparticles (LNPs), offer safer alternatives to viral vectors for gene and cell therapies. These advancements enhance delivery efficiency and therapeutic outcomes, paving the way for personalized medicine.

Keywords:
CAR-T cell therapyantisense oligonucleotides (ASOs)gene deliverygene editinggene therapylipid nanoparticles (LNPs)mRNA therapeuticsnon-viral vectorsregenerative medicinesiRNAtransfection

More Related Videos

Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates
06:10

Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates

Published on: May 9, 2025

433
High-Throughput DNA Plasmid Multiplexing and Transfection Using Acoustic Nanodispensing Technology
13:27

High-Throughput DNA Plasmid Multiplexing and Transfection Using Acoustic Nanodispensing Technology

Published on: August 8, 2019

9.0K

Related Experiment Videos

Last Updated: Sep 11, 2025

Small RNA Transfection in Primary Human Th17 Cells by Next Generation Electroporation
10:15

Small RNA Transfection in Primary Human Th17 Cells by Next Generation Electroporation

Published on: April 13, 2017

8.0K
Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates
06:10

Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates

Published on: May 9, 2025

433
High-Throughput DNA Plasmid Multiplexing and Transfection Using Acoustic Nanodispensing Technology
13:27

High-Throughput DNA Plasmid Multiplexing and Transfection Using Acoustic Nanodispensing Technology

Published on: August 8, 2019

9.0K

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Therapeutic Delivery

Background:

  • Transfection is crucial for advanced therapies like gene therapy, mRNA treatments, CAR-T cell therapy, and regenerative medicine.
  • While viral vectors are common, non-viral systems, such as lipid nanoparticles (LNPs), provide safer and more adaptable alternatives.
  • This research reviews novel non-viral transfection technologies to enhance delivery efficiency and therapeutic results.

Purpose of the Study:

  • To explore and synthesize current advancements in non-viral transfection technologies.
  • To evaluate the mechanisms, benefits, and drawbacks of diverse non-viral delivery systems.
  • To compare the performance of these systems for clinical translation.

Main Methods:

  • Literature review of academic search engines and online databases (PubMed, Scopus, Google Scholar).
  • Focus on lipid nanoparticles, biodegradable polymers, electroporation, peptide carriers, and microfluidic platforms.
  • Comparative analysis of transfection efficiency, cellular uptake, biocompatibility, and clinical potential.

Main Results:

  • Non-viral systems excel in delivering mRNA, siRNA, and oligonucleotides, especially in clinical settings.
  • Biodegradable polymers and peptide systems show potential for improved biocompatibility and targeted delivery.
  • Electroporation and microfluidics offer precise control, enhancing reproducibility and scalability for gene delivery.

Conclusions:

  • Non-viral transfection technologies are key to advancing gene and cell-based therapies.
  • Lipid nanoparticles and emerging platforms like microfluidics and polymers offer safer, flexible alternatives to viral vectors.
  • These innovations are vital for optimizing therapeutic efficacy in personalized medicine, immunotherapy, and regenerative treatments.