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Hydrogel Viscoelasticity Modulates Cell Nascent Extracellular Matrix Deposition.

Matthew L Tan1, Avinava Roy1,2,3, Eleanor M Plaster4

  • 1Department of Materials Science and Engineering, University of Michigan, Ann Arbor, Michigan, USA.

Macromolecular Rapid Communications
|August 14, 2025
PubMed
Summary

This study shows that high-viscosity hydrogels increase nascent extracellular matrix (ECM) deposition by cells, influencing cell-environment interactions. This finding offers new insights into how cells remodel their microenvironment.

Keywords:
extracellular matrix depositionhydrogelsmechanotransductionviscoelasticity

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Area of Science:

  • Biomaterials Science
  • Cell Biology
  • Tissue Engineering

Background:

  • Polymeric hydrogels mimic native tissue extracellular matrix (ECM) mechanical properties.
  • Cells remodel their microenvironment by depositing nascent ECM, which influences mechanical signaling.
  • Understanding nascent ECM's role in cell response to time-dependent mechanics is crucial.

Purpose of the Study:

  • To investigate how hydrogel viscosity affects nascent ECM deposition and remodeling.
  • To explore the relationship between hydrogel mechanics and cell function.
  • To develop an interpenetrating polymer network for independent control of hydrogel properties.

Main Methods:

  • Developed an interpenetrating polymer network hydrogel system.
  • Independently controlled viscous and elastic properties of hydrogels.
  • Cultured cells on hydrogels with varying viscosity and measured ECM deposition and remodeling.

Main Results:

  • Cells on high-viscosity hydrogels deposited more nascent ECM.
  • Increased nascent ECM deposition correlated with enhanced hydrogel remodeling.
  • Nascent ECM deposition on high-viscosity hydrogels was independent of intracellular contractility.

Conclusions:

  • Hydrogel viscosity directly influences nascent ECM deposition by cells.
  • Nascent ECM remodeling is linked to hydrogel viscosity, impacting cell-hydrogel interactions.
  • Findings provide insights into cell mechanobiology and biomaterial design.