What Is Regulating Chironomid Populations? The Influence of Food Supply and Interference Competition on Development...

Density-dependent processes are important for a fundamental understanding of population regulation, as well as for understanding responses to and recovery from stressors. While exploitative competition is well-studied, interference competition is rather difficult to investigate, but it has been regularly observed to occur in many aquatic insect populations. We conducted laboratory experiments with the non-biting midge Chironomus riparius (Diptera: Chironomidae) to investigate the impact of different combinations of food supply and larval densities on development and mortality at a constant temperature of 20°C. The chosen two-factorial experimental design allowed a separate evaluation of exploitative (food) and interference (mortality) competition across a gradient of larval densities. The use of different vessel sizes between 50 cm² and 600 cm² made it possible to quantify the functional response at different food densities. To test mechanistic explanations for the statistically significant empirical relationships found in this study and to predict density-dependent processes, we used a dynamic process-oriented modeling approach. We extended a recently developed DEB-IBM full life cycle model for C. riparius and successfully applied it under variable food conditions at the population level under laboratory conditions. Our study showed that chironomid development and reproduction are primarily dependent on food supply, whereas larval density drives the density-dependent mortality rate. The interaction of food availability and interference competition determined the effective mortality over time. Killing by conspecifics was the most likely mechanism responsible for the intraspecific mortality of the larval stages. Combining data generated using a tailor-made experimental design with a mechanistic model provided insights into and quantified regulation mechanisms of chironomid populations, allowing future uses of this information in the context of population-level risk assessment from exposure to chemicals.