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Updated: Sep 11, 2025

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography
Published on: January 15, 2013
Optoretinography with actively stabilized adaptive optics optical coherence tomography
Jason H Wong1, Shangbang Luo1, Zohreh Hosseinaee1
1Herbert Wertheim School of Optometry and Vision Science, University of California Berkeley, Berkeley, CA 94720-2020, USA.
Abstract:
Optoretinography (ORG) is the optical measurement of changes in the retina in response to light stimulation. Adaptive optics optical coherence tomography (AOOCT) records photoreceptor ORGs by measuring the physical changes in their outer segment lengths in response to light stimulation. The main difficulty in recording these nanometer-scale changes is constant eye motion. Typically, fast volume acquisitions are used with offline spatial registration to compensate for the effect of eye motion. Here, we present an alternate solution whereby an adaptive optics scanning light ophthalmoscope (AOSLO) is used to measure the eye motion and actively guide the AOOCT beam to compensate for eye motion in real time. This system's cellular-scale tracking offers unparalleled control over scanning raster size and shape, allowing for high-speed (up to 100 kHz) ORG acquisition from targeted locations. We validate the method by comparing cone classifications against those made with an established ORG approach.
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