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Related Concept Videos

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation01:26

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
There are three main types of inductively coupled plasma atomic emission spectroscopy  (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used.

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Confocal Absorbance-Activated Droplet Sorting (cAADS) for Enzyme Engineering.

Abdi Mirgissa Kaba1, Sébastien Gounel1, Thomas Beneyton1

  • 1Centre de Recherche Paul Pascal (CRPP), CNRS UMR 5031, Univ. Bordeaux, 115 Avenue du Docteur Schweitzer, Pessac, 33600, France.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|August 14, 2025
PubMed
Summary
This summary is machine-generated.

Confocal Absorbance-Activated Droplet Sorting (cAADS) enables sensitive, high-throughput screening of enzyme variants using absorbance detection. This microfluidic system accelerates enzyme engineering by overcoming limitations of fluorescence-based methods.

Keywords:
absorbance‐activated droplet screeningbilirubin oxidasedirected evolutiondroplet‐based microfluidicshigh‐throughput screening

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Area of Science:

  • Biotechnology
  • Chemical Engineering
  • Microfluidics

Background:

  • Directed evolution requires efficient screening of enzyme variants.
  • Droplet microfluidics offers high-throughput screening but is limited by assay types.
  • Fluorescence-based assays are common, but absorbance assays cover more enzymatic systems.

Purpose of the Study:

  • To develop a microfluidic system for sensitive, high-throughput absorbance-based screening of enzyme variants.
  • To overcome the sensitivity and throughput limitations of existing droplet microfluidic screening methods.

Main Methods:

  • Development of a confocal Absorbance-Activated Droplet Sorting (cAADS) system.
  • Utilizing picoliter-scale water-in-oil emulsions as microcompartments for absorbance measurements.
  • Demonstration with Bilirubin Oxidase (BOD) and Glucose Oxidase variants.

Main Results:

  • Achieved sensitive absorbance measurements at ultrahigh throughput (5.4 kHz) with 10 pL droplets.
  • Enabled sorting of 50 pL droplets at frequencies up to 2.6 kHz.
  • Demonstrated 99% sorting efficiency for active BOD variants and versatility with Glucose Oxidase.

Conclusions:

  • The cAADS system significantly enhances microfluidic screening capabilities for enzyme engineering.
  • This technology expands the applicability of droplet microfluidics to absorbance-based assays.
  • cAADS offers a versatile and efficient platform for directed evolution and enzyme discovery.