Pseudomonas bambusae sp.nov., an Aniline Blue-Decolorizing Bacterium Isolated from Decaying Round Bamboo Culms

A novel bacterial strain, designated RC10^T, was isolated from decaying round bamboo culms. Phylogenetic and sequence similarity analysis of 16S rRNA gene sequences indicated that strain RC10^T was grouped with members of the genus Pseudomonas, with the highest similarity of 98.4% to Pseudomonas lutea DSM 17257^T, followed by Pseudomonas kuykendallii DSM 25107^T (98.2%). The average nucleotide identity (ANI) and in silico DNA-DNA hybridization (dDDH) values between strain RC10^T and species with validly published name of the genus Pseudomonas were less than 84.93% and 20.50%, respectively. Strain RC10^T was rod-shaped, Gram-stain-negative, and exhibited optimal growth at 28 °C, pH 6.5, and 0% NaCl (w/v). Notably, strain RC10^T showed a high capacity for aniline blue decolorization, with the decolorization rate reaching 93.99% after 48 h of incubation. Genes encoding laccase, DyP-type peroxidases, NADH-dependent FMN reductase, and NAD(P)H dehydrogenase were found in the genome of strain RC10^T, which may play crucial roles in aniline blue decolorization. Chemical taxonomic analysis indicated that the polar lipids profile of strain RC10^T consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, three uncharacterized phospholipids, two uncharacterized glycolipids, and three uncharacterized lipids. Summed feature 8 (C_18:1 ω7c and/or C_18:1 ω6c), summed feature 3 (C_16:1 ω7c and/or C_16:1 ω6c), and C_16:0 was determined as the main fatty acids. Ubiquinone 9 was identified as the sole respiratory quinone. The G+C content of the genomic DNA of strain RC10^T was 60.1%. Based on phenotypic, chemotaxonomic, and genotypic features revealed in this investigation, strain RC10^T represents a novel species in the genus Pseudomonas, and the proposed name for this novel species is Pseudomonas bambusae sp. nov. The type strain is RC10^T (= MCCC 1K08846^T = JCM 36624^T).