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Published on: March 11, 2020
SlGSTU43 and SlERF-B12 Genes Are Involved in SlMYB15-Regulated Redox Homeostasis to Enhance Cold Tolerance in Tomato
Luyue Zhang1, Xuyang Du1, Aowei Wang1
1Henan International Joint Laboratory of Crop Gene Resources and Improvements, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, China.
Abstract:
Cold stress is a major abiotic factor limiting plant growth and productivity. MYB transcription factors are important regulators of stress responses. Our previous study identified SlMYB15 as a positive regulator of cold tolerance in tomato. However, their downstream regulatory mechanisms remained unclear. Herein, subcellular localization confirmed that SlMYB15 is a nuclearly localized transcription factor. Transcriptomic analysis revealed that SlMYB15 modulates genes involved in redox processes, hormone signaling, and glutathione metabolism under cold stress. SlMYB15 upregulated GSTU43, ERF-B12, CYP76A2-like, and CYP81D1-like, reducing ROS accumulation by enhancing antioxidant enzyme activities and modulating RBOH1-dependent H2O2 signaling. Silencing GSTU43 or ERF-B12 impaired the cold tolerance in SlMYB15-overexpressing plants. Furthermore, SlMYB15 activated the expression of GSTU43 and ERF-B12 by directly binding to the promoter regions. Collectively, these findings suggest that SlMYB15 enhances cold tolerance by regulating key redox-related genes, providing new insights into MYB-mediated stress resistance in tomato.
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