Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Wide Dynamic-Range Polarization-Controlled SPR Imaging Method for High-Throughput Biomolecular Detection.

Analytical chemistry·2026
Same author

Oligo-cyanobacterial microalgae-bacteria granular sludge for mitigating cyanotoxin risk: Cultivation, characteristics, and formation mechanism.

Journal of environmental management·2026
Same author

LXN-THBS2 Signaling Axis Regulates Hepatic Stellate Cell Activation and Promotes the Development of Liver Fibrosis.

Frontiers in bioscience (Landmark edition)·2026
Same author

Cinnamaldehyde suppresses ovarian cancer progression by activating ROS-mediated apoptosis and mitophagy.

Biochemical pharmacology·2025
Same author

Portable point-of-care testing of food glutathione enabled by a high-performance sulfur-vacancy FeS mimetic enzyme.

Food chemistry·2025
Same author

Prevalence and Genotype Distribution of Human Papillomavirus in 23,495 Cases in Guangzhou City, Guangdong Province, China.

Current microbiology·2025

Related Experiment Video

Updated: Sep 11, 2025

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance SPR
09:35

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance SPR

Published on: November 29, 2014

22.9K

Algorithm assisted comprehensive optimization of SPR sensors towards single molecule detection.

Tingting Liu1, Shiqi Hu2, Shilei Ding3

  • 1Department of Optoelectronic Engineering, College of Physics and Optoelectronic Engineering, Jinan University, Guangzhou, 510632, PR China; Guangdong Provincial Key Laboratory of Optical Fiber Sensing and Communications, Jinan University, Guangzhou, 510632, PR China; Guangdong Provincial Engineering Research Center for Electrical and Intelligent Control, Zhuhai, Guangdong 519070, China.

Biosensors & Bioelectronics
|August 14, 2025
PubMed
Summary

This study enhances surface plasmon resonance (SPR) biosensors for ultra-sensitive single-molecule detection. Multi-objective optimization significantly improves sensitivity and lowers detection limits for biochemical analysis.

Keywords:
AlgorithmComprehensive optimizationLow LODSPR biosensors

More Related Videos

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms
15:27

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms

Published on: April 17, 2017

21.0K
Using Extraordinary Optical Transmission to Quantify Cardiac Biomarkers in Human Serum
09:23

Using Extraordinary Optical Transmission to Quantify Cardiac Biomarkers in Human Serum

Published on: December 13, 2017

6.4K

Related Experiment Videos

Last Updated: Sep 11, 2025

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance SPR
09:35

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance SPR

Published on: November 29, 2014

22.9K
Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms
15:27

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms

Published on: April 17, 2017

21.0K
Using Extraordinary Optical Transmission to Quantify Cardiac Biomarkers in Human Serum
09:23

Using Extraordinary Optical Transmission to Quantify Cardiac Biomarkers in Human Serum

Published on: December 13, 2017

6.4K

Area of Science:

  • Biochemistry
  • Biosensing Technology
  • Nanotechnology

Background:

  • Single-molecule detection is crucial for understanding molecular interactions.
  • Surface plasmon resonance (SPR) biosensors offer label-free, rapid monitoring but struggle with low analyte concentrations.
  • Detecting single molecules requires highly sensitive and optimized biosensing platforms.

Purpose of the Study:

  • To enhance the performance of label-free SPR biosensors for single-molecule detection.
  • To improve key sensing metrics like sensitivity, figure of merit (FOM), and resonant dip depth.
  • To mitigate processing errors through optimized design parameter selection.

Main Methods:

  • A multi-objective optimization strategy was employed to enhance SPR biosensor performance.
  • K-means clustering was used to identify optimal design parameters.
  • The optimized SPR biosensor was validated using mouse immunoglobulin G (IgG).

Main Results:

  • Significant improvements were observed in refractive index sensitivity (+230.22%), FOM (+110.94%), and depth of resonant dip (DFOM, +90.85%).
  • Achieved a bulk refractive index sensitivity of 24,482.86 nm/RIU.
  • Demonstrated a broad linear dynamic range (fg/mL to μg/mL) and a low limit of detection (LOD) of 54 ag/mL (0.36 aM).

Conclusions:

  • The multi-objective particle swarm optimization strategy provides an efficient method for SPR biosensor optimization.
  • This approach significantly enhances ultra-sensitive single-molecule detection capabilities.
  • The optimized SPR biosensor exhibits a very low LOD, suitable for various biochemical applications.