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Updated: Sep 11, 2025

Anti-virulent Disruption of Pathogenic Biofilms using Engineered Quorum-quenching Lactonases
Published on: January 1, 2016
Efficiency and mechanism of biological nitrogen removal mediated by endogenous signaling molecules based on quorum
Lilong Yan1, Xu Song1, Xin Wang1
1College of Resource and Environment, Northeast Agricultural University, Harbin 150030 China.
Abstract:
To elucidate the role of endogenous signaling molecules (ESM) in biological denitrification, the dynamic changes in sludge denitrification efficiency, activation energy, electron transport characteristics, related enzyme activity and extracellular polymeric substances (EPS) composition in the short-term and long-term presence of signaling molecules (SM) hydrolases were investigated in this study. Furthermore, the responses of the microbial community structure and related functional genes were analysed. The presence of ESM could increase the denitrification activity of sludge by 9-25 %, promote the secretion of protein, polysaccharide, and humic acid in EPS, and reduce the activation energy of denitrification. Moreover, the presence of ESM increased the electron transport system activity and nitrate reductase activity by 33.73 ± 3.99 % and 58.3 ± 4.46 %, respectively. Moreover, the presence of ESM upregulated the abundance of genes related to EPS synthesis and nitrate nitrogen transporters, increased microbial electron transfer efficiency, and enriched nitrogen-related transformation microorganisms such as unclassified_o_Saccharimonadales, Micropruina, and unclassified_f_Enterobacteriaceae. The long-term presence of SM hydrolases increased the denitrification activity of sludge by promoting the expression of functional genes related to nitrogen conversion and transfer, increasing the electron transfer capacity. This study provides novel insights into the biological denitrification process mediated by SM.
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