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Abstract:
The ambiguous location of photosynthetic carboxylases of mesophyll and bundle sheath chloroplasts of sorghum was investigated after successful homogeneous preparation. The phosphoenol pyruvate carboxylase was found as a particulate enzyme in the mesophyll cell chloroplasts and ribulose 1,5-biphosphate carboxylase in the stroma of the bundle sheath cell chloroplasts. Extensive characterization was carried out on these 2 enzymes for better understanding of the enzyme action.