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High-resolution Thermal Micro-imaging Using Europium Chelate Luminescent Coatings
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Single-Molecule Vibrational Thermometry.

Philip A Kocheril1, Dongkwan Lee1, Noor Naji1

  • 1Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, United States.

The Journal of Physical Chemistry. B
|August 15, 2025
PubMed
Summary
This summary is machine-generated.

We developed Boltzmann-edge vibrational thermometry (BET) for calibration-free temperature measurements. This sensitive molecular thermometer works in biological samples and can identify local environments.

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Area of Science:

  • Biophysics
  • Chemical Physics
  • Molecular Imaging

Background:

  • Molecular thermometers are crucial for in situ temperature measurements.
  • Existing methods often require calibration and lack sensitivity in complex environments.

Purpose of the Study:

  • To introduce Boltzmann-edge vibrational thermometry (BET) as a novel, calibration-free method for temperature measurement.
  • To demonstrate the applicability of BET in biological samples with high sensitivity.

Main Methods:

  • Utilizing anti-Stokes fluorescence to detect the relative population of vibrationally excited molecules.
  • Applying the Boltzmann distribution for calibration-free temperature reporting.
  • Employing mid-infrared absorption to modulate and characterize vibrational temperature for BET fingerprinting.

Main Results:

  • Demonstrated BET microscopy's compatibility with biological samples.
  • Achieved single-molecule sensitivity using BET.
  • Showcased the ability to characterize local environments via BET fingerprinting.

Conclusions:

  • BET provides a foundation for sensitive, calibration-free vibrational thermometry in biological imaging.
  • This technique allows for detailed characterization of local environments through molecular vibrations.