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Updated: Jul 7, 2026
Isolation, Culture, and Genetic Engineering of Mammalian Primary Pigment Epithelial Cells for Non-Viral Gene Therapy
Published on: February 26, 2021
Transcriptomic analysis reveals the impact of Rutin on the proliferation, barrier function, and transcription of
Binbin Wang1, Lele Huo1, Wanru Tian1
1Jiangsu Key Laboratory of Sericultural and Animal Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, 212100, China.
Abstract:
The gastrointestinal health of livestock is essential for the agricultural sector. Rutin, a natural flavonoid extract from plants, exhibits antioxidant and anti-inflammatory properties, alongside a protective impact on the intestinal tract of pigs. However its influence on the proliferation, migration, and cellular junctions of IPEC-J2 cells remains unexplored. This study involved treating IPEC-J2 cells with varying concentrations of rutin. It was observed that after 24 h of treatment with 200 µM rutin, cell viability was significantly increased, along with significant upregulation in the mRNA expression levels of proliferation-related genes (CCNE2, CDK4, CDK2, CCNDB, and PCNA) and tight junction genes (ZO-1, Occludin, and Claudin-1). Additionally, the expression of proliferation-related PCNA protein was significantly increased. Transcriptome sequencing revealed that rutin treatment impacted the overall transcription profile of IPEC-J2 cells, altering the expression of genes involved in critical physiological processes such as redox balance, cell proliferation, and tight junctions maintenance. Consequently, rutin demonstrated a significant enhancement in the proliferation of IPEC-J2 cells and improved the intestinal barrier function in pigs, offering valuable insights for sustaining pig intestinal health in swine husbandry.
