Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A one-step immunoassay of Tau protein based on flow cytometric counting of target-induced nanoaggregates.

Chemical communications (Cambridge, England)·2026
Same author

Precise Artificial Intelligence Nanomotor Tracking-Powered Amplification-Free Digital MicroRNA Sensing.

Analytical chemistry·2026
Same author

Single Microbead Floating/Sinking-Enabled Digital Biosensing.

Nano letters·2026
Same author

Real-Time Digital Micromotor Tracking-Enabled Ultrasensitive Immunoassay.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Enzyme inhibition-enabled CRISPR/Cas12a biosensing system for heparin-related non-nucleic acid biomarkers.

Chemical communications (Cambridge, England)·2025
Same author

DNA Hybridization-Accelerated Programmable Immuno-Aggregation of Microbeads Enabling Mix-and-Read Flow Cytometric Protein Analysis.

Analytical chemistry·2025

Related Experiment Video

Updated: Sep 11, 2025

Counting Proteins in Single Cells with Addressable Droplet Microarrays
12:25

Counting Proteins in Single Cells with Addressable Droplet Microarrays

Published on: July 6, 2018

8.6K

Intelligent Visualization of Single Microbead Bubbling Enables Attomolar Protein Quantification.

Jingjing Shi1, Zuhua Yu2, Yuanyuan Sun3

  • 1Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry & Chemical Engineering, Shaanxi Normal University, Xi'an, Shaanxi Province 710119, P. R. China.

Analytical Chemistry
|August 15, 2025
PubMed
Summary

This study introduces a portable, cellphone-based biosensor for ultrasensitive protein quantification. The novel single microbead (SMB) bubbling mechanism enables low-cost, rapid disease screening at the point-of-care.

More Related Videos

Calibration-free In Vitro Quantification of Protein Homo-oligomerization Using Commercial Instrumentation and Free, Open Source Brightness Analysis Software
08:22

Calibration-free In Vitro Quantification of Protein Homo-oligomerization Using Commercial Instrumentation and Free, Open Source Brightness Analysis Software

Published on: July 17, 2018

7.4K
A Microfluidic System with Surface Patterning for Investigating Cavitation Bubble(s)–Cell Interaction and the Resultant Bioeffects at the Single-cell Level
11:14

A Microfluidic System with Surface Patterning for Investigating Cavitation Bubble(s)–Cell Interaction and the Resultant Bioeffects at the Single-cell Level

Published on: January 10, 2017

11.8K

Related Experiment Videos

Last Updated: Sep 11, 2025

Counting Proteins in Single Cells with Addressable Droplet Microarrays
12:25

Counting Proteins in Single Cells with Addressable Droplet Microarrays

Published on: July 6, 2018

8.6K
Calibration-free In Vitro Quantification of Protein Homo-oligomerization Using Commercial Instrumentation and Free, Open Source Brightness Analysis Software
08:22

Calibration-free In Vitro Quantification of Protein Homo-oligomerization Using Commercial Instrumentation and Free, Open Source Brightness Analysis Software

Published on: July 17, 2018

7.4K
A Microfluidic System with Surface Patterning for Investigating Cavitation Bubble(s)–Cell Interaction and the Resultant Bioeffects at the Single-cell Level
11:14

A Microfluidic System with Surface Patterning for Investigating Cavitation Bubble(s)–Cell Interaction and the Resultant Bioeffects at the Single-cell Level

Published on: January 10, 2017

11.8K

Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Point-of-Care Diagnostics

Background:

  • Accurate quantification of low-abundance proteins in biofluids is crucial for point-of-care testing and disease monitoring.
  • Existing methods often lack portability, affordability, and the required sensitivity for early disease detection.

Purpose of the Study:

  • To develop a facile, low-cost, and highly sensitive portable immunoassay platform for protein analysis.
  • To enable ultrasensitive protein detection down to attomolar (aM) levels using a novel signal readout.

Main Methods:

  • A cellphone-based portable immunoassay platform utilizing a single microbead (SMB) as an immunocarrier.
  • Platinum nanoparticle (PtNP)-enhanced immunocomplexes on the SMB catalyze oxygen bubble formation.
  • A cellphone-attached lens and image analysis algorithm for bubble imaging and quantification.

Main Results:

  • The SMB bubbling visualization mechanism achieved superenrichment of target-mediated immunocomplexes, significantly boosting sensitivity.
  • Ultrasensitive protein detection down to aM levels was demonstrated.
  • An all-in-one cellphone application streamlined the entire detection process.

Conclusions:

  • The proposed intelligent platform offers a highly sensitive and cost-effective method for critical biomarker testing.
  • This technology paves a new way for cellphone-enabled portable biosensing and in-field disease screening.